Increased microglia proliferation separates pilocytic astrocytomas from diffuse astrocytomas: A double labeling study

被引：0
作者：
Klein R. [1 ]
Roggendorf W. [1 ]
机构：
[1] Institute of Pathology, Department of Neuropathology, University of Würzburg, 97080 Würzburg
来源：
Acta Neuropathologica | 2001年 / 101卷 / 3期
关键词：
Brain neoplasm; Glioma; Microglia; Pilocytic astrocytoma; Proliferation;
D O I：
10.1007/s004010000286
中图分类号：
学科分类号：
摘要：
It is not known how many non-tumorous cells in gliomas contribute to the proliferation rate. We investigated the proliferative activity of microglia in an immunohistochemical double-labeling study of pilocytic astrocytomas and astrocytomas WHO grade II-IV using the antibodies MIB-1 (Ki67) as proliferation-marker and Ki-M1P (CD68) as microglia marker. We found the highest indices of proliferating microglia in pilocytic astrocytomas with an average rate of 32% (±6.8) of all proliferating cells. In contrast, the proliferation indices of microglia were lowest in fibrillary astrocytomas with 8.6% (±2.5) of all proliferating cells. In anaplastic astrocytomas and glioblastomas the percentage of proliferating microglia showed a slight increase to 8.8% (±3.6) and 13.4% (±8.7), respectively. We conclude that microglial cells in astrocytic brain tumors proliferate and show different proliferative activities at different grades of malignancy with the highest rates of proliferating microglia especially in pilocytic astrocytomas. Thus, the proliferation rate does not solely reflect the proliferation of tumor cells, but also of non-tumorous cells. This should be considered in particular when proliferation rates are used as a criterion for prognosis and grading of pilocytic astrocytomas.
引用
收藏
页码：245 / 248
页数：3
相关论文
共 24 条
  • [1] Amat J.A., Ishiguro H., Nakamura K., Norton W.T., Phenotypic diversity and kinetics of proliferating microglia and astrocytes following cortical stab wounds, Glia, 16, pp. 368-382, (1996)
  • [2] Burger P.C., Scheithauer B.W., Tumors of the central nervous system, pp. 77-96, (1994)
  • [3] Dobbertin A., Schmid P., Gelman M., Glowinski J., Mallat M., Neurons promote macrophage proliferation by producing transforming growth factor-β2, J Neurosci, 17, pp. 5305-5315, (1997)
  • [4] Ganter S., Northoff H., Mannel D., Gebicke-Harter P.J., Growth control of cultured microglia, J Neurosci Res, 33, pp. 218-230, (1992)
  • [5] Giannini C., Scheithauer B.W., Burger P.C., Christensen M.R., Wollan P.C., Sebo T.J., Forsyth P.A., Hayostek C.J., Cellular proliferation in pilocytic and diffuse astrocytomas, J Neuropathol Exp Neurol, 58, pp. 46-53, (1999)
  • [6] Giometto B., Bozza F., Faresin F., Alessio L., Mingrino S., Tavolato B., Immune infiltrates and cytokines in gliomas, Acta Neurochir, 138, pp. 50-56, (1996)
  • [7] Ho D.M., Wong T.T., Hsu C.Y., Ting L.T., Chiang H., MIB-1 labeling index in nonpilocytic astrocytoma of childhood. A study of 101 cases, Cancer, 82, pp. 2459-2466, (1998)
  • [8] Hsu D.W., Louis D.N., Efird J.T., Hedley-Whyte E.T., Use of MIB-1 (Ki-67) immunoreactivity in differentiating grade II and grade III gliomas, J Neuropathol Exp Neurol, 56, pp. 857-865, (1997)
  • [9] Ilyin S.E., Gonzalez-Gomez I., Gilles F.H., Plata-Salaman C.R., Interleukin-1 α (IL-1α), IL-1β, IL-1 receptor type I, IL-1 receptor antagonist, and TGF-β1 mRNAs in pediatric astrocytomas, ependymomas and primitive neuroectodermal tumors, Mol Chem Neuropathol, 33, pp. 125-137, (1998)
  • [10] Kleihues P., Burger P.C., Scheithauer B.W., Histological Typing of Tumours of the Central Nervous System, (1993)
123