Diagnosis support of sickle cell anemia by classifying red blood cell shape in peripheral blood images

被引:0
作者
Wilkie Delgado-Font
Miriela Escobedo-Nicot
Manuel González-Hidalgo
Silena Herold-Garcia
Antoni Jaume-i-Capó
Arnau Mir
机构
[1] Universidad de Oriente,Departamento de Computación, Facultad de Ciencias Naturales y Exactas
[2] Universitat de les Illes Balears,Balearic Islands Health Research Institute (IdISBa), Soft Computing, Image Processing and Aggregation (SCOPIA) Research Group, Department of Mathematics and Computer Science
[3] Universitat de les Illes Balears,Research Institute of Health Sciences (IUNICS), Computer Graphics and Vision and AI Group (UGiVIA), Department of Mathematics and Computer Science
[4] Universitat de les Illes Balears,Balearic Islands Health Research Institute (IdISBa), Computational Biology and Bioinformatics (BIOCOM) Research Group, Department of Mathematics and Computer Science
来源
Medical & Biological Engineering & Computing | 2020年 / 58卷
关键词
Red blood cell; Sickle cell anemia; Deformation; Cellular classification; Medical imaging; Image processing; Peripheral blood image;
D O I
暂无
中图分类号
学科分类号
摘要
Red blood cell (RBC) deformation is the consequence of several diseases, including sickle cell anemia, which causes recurring episodes of pain and severe pronounced anemia. Monitoring patients with these diseases involves the observation of peripheral blood samples under a microscope, a time-consuming procedure. Moreover, a specialist is required to perform this technique, and owing to the subjective nature of the observation of isolated RBCs, the error rate is high. In this paper, we propose an automated method for differentially enumerating RBCs that uses peripheral blood smear image analysis. In this method, the objects of interest in the image are segmented using a Chan-Vese active contour model. An analysis is then performed to classify the RBCs, also called erythrocytes, as normal or elongated or having other deformations, using the basic shape analysis descriptors: circular shape factor (CSF) and elliptical shape factor (ESF). To analyze cells that become partially occluded in a cluster during sample preparation, an elliptical adjustment is performed to allow the analysis of erythrocytes with discoidal and elongated shapes. The images of patient blood samples used in the study were acquired by a clinical laboratory specialist in the Special Hematology Department of the “Dr. Juan Bruno Zayas” General Hospital in Santiago de Cuba. A comparison of the results obtained by the proposed method in our experiments with those obtained by some state-of-the-art methods showed that the proposed method is superior for the diagnosis of sickle cell anemia. This superiority is achieved for evidenced by the obtained F-measure value (0.97 for normal cells and 0.95 for elongated ones) and several overall multiclass performance measures. The results achieved by the proposed method are suitable for the purpose of clinical treatment and diagnostic support of sickle cell anemia.
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页码:1265 / 1284
页数:19
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