Inheritance Characteristics of Microsatellite DNA Loci in Experimental Families of Japanese Flounder Paralichthys olivaceus

被引:0
作者
Masashi Sekino
Motoyuki Hara
机构
[1] Tohoku National Fisheries Research Institute,
[2] Shinhama,undefined
[3] Shiogama,undefined
[4] Miyagi,undefined
[5] 985-0001,undefined
[6] Japan,undefined
[7] National Research Institute of Aquaculture,undefined
[8] Nansei,undefined
[9] Watarai,undefined
[10] Mie,undefined
[11] 516-0193,undefined
[12] Japan,undefined
来源
Marine Biotechnology | 2001年 / 3卷
关键词
Key words: microsatellite DNA loci, Japanese flounder, Paralichthys olivaceus, Mendelian inheritance.;
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学科分类号
摘要
Allele segregating patterns of microsatellite DNA loci in 5 experimental families of Japanese flounder Paralichthys olivaceus and genotype frequencies for deviation from Hardy-Weinberg expectations (HWE) in natural P. olivaceus populations were studied to assess inheritability. Of the 12 microsatellite loci examined, 1 locus had a possibility of scoring errors of heterozygous individuals caused by unreproducible polymerase chain reaction amplifications of a particular allele. At the remaining 11 loci, almost all of alleles were segregated according to Mendelian transmission, and observed genotype frequencies in natural populations were consistent with HWE. The results demonstrated here would provide useful information supporting the suitability of these microsatellite loci as inheritable P. olivaceus genetic markers.
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页码:310 / 315
页数:5
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