Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci

[1] Mali P(2013)RNA-guided human genome engineering via Cas9 Science 339 823-826

[2] Yang L(2015)Highly efficient CRISPR/HDR-mediated knock-in for mouse embryonic stem cells and zygotes Biotechniques 59 201-202, 204, 206–208

[3] Esvelt KM(2014)Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system Mamm Genome Off J Int Mamm Genome Soc 25 327-334

[4] Aach J(2018)Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos Nat Biotechnol 36 632-637

[5] Guell M(2015)Efficient CRISPR/Cas9-mediated genome editing in mice by zygote electroporation of nuclease Genetics 200 423-430

[6] DiCarlo JE(2015)Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing Sci Rep 5 11315-215

[7] Norville JE(2017)Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins Genome Biol 18 92-W245

[8] Church GM(2018)Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors Nat Protoc 13 195-W174

[9] Wang B(2018)CLICK: one-step generation of conditional knockout mice BMC Genomics 19 318-271

[10] Li K(2017)Efficient generation of conditional knockout mice via sequential introduction of lox sites Sci Rep 7 7891-1379

[1] Mali P(2013)RNA-guided human genome engineering via Cas9 Science 339 823-826

[2] Yang L(2015)Highly efficient CRISPR/HDR-mediated knock-in for mouse embryonic stem cells and zygotes Biotechniques 59 201-202, 204, 206–208

[3] Esvelt KM(2014)Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system Mamm Genome Off J Int Mamm Genome Soc 25 327-334

[4] Aach J(2018)Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos Nat Biotechnol 36 632-637

[5] Guell M(2015)Efficient CRISPR/Cas9-mediated genome editing in mice by zygote electroporation of nuclease Genetics 200 423-430

[6] DiCarlo JE(2015)Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing Sci Rep 5 11315-215

[7] Norville JE(2017)Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins Genome Biol 18 92-W245

[8] Church GM(2018)Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors Nat Protoc 13 195-W174

[9] Wang B(2018)CLICK: one-step generation of conditional knockout mice BMC Genomics 19 318-271

[10] Li K(2017)Efficient generation of conditional knockout mice via sequential introduction of lox sites Sci Rep 7 7891-1379