Technological breakthroughs in generating transgene-free and genetically stable CRISPR-edited plants

被引:0
作者
Yubing He
Yunde Zhao
机构
[1] Huazhong Agricultural University,National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan)
[2] University of California San Diego,Section of Cell and Developmental Biology
来源
aBIOTECH | 2020年 / 1卷
关键词
CRISPR; Transgene-free; Marker-assisted selection; TKC; Cas9; gene editing;
D O I
暂无
中图分类号
学科分类号
摘要
CRISPR/Cas9 gene-editing technologies have been very effective in editing target genes in all major crop plants and offer unprecedented potentials in crop improvement. A major challenge in using CRISPR gene-editing technology for agricultural applications is that the target gene-edited crop plants need to be transgene free to maintain trait stability and to gain regulatory approval for commercial production. In this article, we present various strategies for generating transgene-free and target gene-edited crop plants. The CRISPR transgenes can be removed by genetic segregation if the crop plants are reproduced sexually. Marker-assisted tracking and eliminating transgenes greatly decrease the time and labor needed for identifying the ideal transgene-free plants. Transgenes can be programed to undergo self-elimination when CRISPR genes and suicide genes are sequentially activated, greatly accelerating the isolation of transgene-free and target gene-edited plants. Transgene-free plants can also be generated using approaches that are considered non-transgenic such as ribonucleoprotein transfection, transient expression of transgenes without DNA integration, and nano-biotechnology. Here, we discuss the advantages and disadvantages of the various strategies in generating transgene-free plants and provide guidance for adopting the best strategies in editing a crop plant.
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页码:88 / 96
页数:8
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