RP-HPLC Analysis of Anti-Parkinson’s Drug l-DOPA Content in Mucuna Species from Indian Subcontinent

被引：4
作者：
Patil R. [1 ,2 ,4 ]
Aware C. [2 ]
Gaikwad S. [3 ]
Rajebhosale M. [2 ]
Bapat V. [2 ]
Yadav S. [3 ]
Jadhav J. [1 ,2 ]
机构：
[1] Department of Biochemistry, Shivaji University, Vidyanagar, Kolhapur, Maharashtra
[2] Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, Maharashtra
[3] Department of Botany, Shivaji University, Vidyanagar, Kolhapur, Maharashtra
[4] Amity Institute of Biotechnology, Amity University, Mumbai, Maharashtra
来源：
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences | 2019年 / 89卷 / 4期
关键词：
Ayurveda; HPLC; l-DOPA; Mucuna; Parkinson’s disease; Shikimic acid pathway;
D O I：
10.1007/s40011-018-01071-9
中图分类号：
学科分类号：
摘要：
Genus Mucuna has tremendous value in the Indian Ayurveda, and the species have been used for the management of diseases including Parkinson’s disease. Unfortunately, species other than M. pruriens are neglected for scientific investigation. In the present study, quantification of anti-Parkinson’s drug l-DOPA content using HPLC from eighteen different Mucuna samples from Indian subcontinent comprising nine species, five varieties of M. pruriens and a commercial Mucuna powder from Zandu Pharmaceutical Ltd. (MZP) is carried out. Seeds of eighteen Mucuna accessions were analyzed for l-DOPA content using RP-HPLC. The l-DOPA content from different samples is compared with commonly used M. pruriens var utilis. Moreover, possible correlation of protein and carbohydrate with l-DOPA synthesis is hypothesized. The authors found varying l-DOPA content in different species and varieties; and higher l-DOPA containing species were M. macrocarpa (11.6 ± 0.21%), M. sanjappae (10.81 ± 0.2%) and M. atropurpurea (10.80 ± 0.1%). Some of the M. pruriens accessions contained higher level of l-DOPA including M. pruriens var hirsuta (10.2 ± 0.15%), M. pruriens var utilis (Manipur) (9.9 ± 0.15%) and M. pruriens var pruriens (Aurangabad) (9.41 ± 0.07 and 9.5 ± 0.12%). The species, M. gigentea had the lowest l-DOPA content (3.68 ± 0.17%). The strong correlation between l-DOPA and carbohydrate content (R2 = 0.7997) was observed, whereas insignificant correlation (R2 = 0.0277) between protein and l-DOPA content was observed. Higher anti-Parkinson’s drug l-DOPA-containing species were found from India which can be explored further for better extraction of this immense drug. The present study opens a new avenue for metabolite modeling in plant for commercial production of l-DOPA at industrial level. © 2019, The National Academy of Sciences, India.
引用
收藏
页码：1413 / 1420
页数：7
相关论文
共 29 条
  • [1] Jadiya P., Khan A., Sammi S., Kaur S., Mir S., Nazir A., Anti-Parkinsonian effects of Bacopa monnieri: insights from transgenic and pharmacological Caenorhabditis elegans models of Parkinson’s disease, Biochem Biophys Res Commun, 413, 4, pp. 605-610, (2011)
  • [2] Thomas B., Flint Beal M., Parkinson’s disease, Hum Mol Genet, 16, pp. 183-194, (2007)
  • [3] Inamdar S.A., Surwase S.N., Jadhav S.B., Bapat V.A., Jadhav J.P., Statistically optimized biotransformation protocol for continuous production of l-DOPA using Mucuna monosperma callus culture, SpringerPlus, 2, pp. 570-578, (2013)
  • [4] Prakash J., Yadav S.K., Chouhan S., Singh S.P., Neuroprotective role of Withania somnifera root extract in maneb–paraquat induced mouse model of parkinsonism, Neurochem Res, 38, 5, pp. 972-980, (2013)
  • [5] Politisa M., Niccolinia F., Serotonin in Parkinson’s disease, Behav Brain Res, 277, pp. 136-145, (2014)
  • [6] Guggenheim Z., Physiol Chem, 88, (1913)
  • [7] Damodaran M., Ramaswamy R., Isolation of l-3: 4-dihydroxyphenylalanine from the seeds of Mucuna pruriens, Biochem J, 31, 12, (1937)
  • [8] Kofman O., Treatment of Parkinson’s disease with l-DOPA: a current appraisal, Can Med Assoc J, 104, 6, pp. 483-487, (1971)
  • [9] Vijayakumari K., Siddhuraju P., Janardhanan K., Effects of different post-harvest treatments on anti-nutritional factors in seeds of the tribal pulse, Mucuna pruriens (L.) DC, Int J Food Sci Nutr, 47, 3, pp. 263-272, (1996)
  • [10] Aguiyi J.C., Igweh A.C., Egesie U.G., Leoncini R., Studies on possible protection against snake venom using Mucuna pruriens protein immunization, Fitoterapia, 70, 1, pp. 21-24, (1999)
123