Effects of oxidized low density lipoprotein on the expression and function of ABCA1 in macrophages

被引：0
作者：
Li Yinghon
Bi Hao
Wu Fan
Zong Yiqiang
Wang Yan
Qu Shen
机构：
[1] Huazhong University of Science and Technology,Department of Biochemistry & Molecular Biology, Tongji Medical College
来源：
Current Medical Science | 2005年 / 25卷 / 2期
关键词：
ATP binding cassette transporter A1; cholesterol efflux; low density lipoprotein; gene expression regulation;
D O I：
10.1007/BF02873552
中图分类号：
学科分类号：
摘要：
In the present study, we examined the regulation of the expression and function of ABCA1 by modified LDL (ox-LDL)in vitro. After incubation with apoA-I for 24 h, RAW264. 7 cells effluxed 37.65% cholesterol loaded by acetyl LDL (ac-LDL), and 9.78% cholesterol in ox-LDL group. The level of ABCA1 mRNA increased about three times either when cells were incubated with 100 μg/mL ac-LDL or with 100 μg/mL ox-LDL. However, the level of ABCA1 protein rose by 1.57 times in ac-LDL group and 1.26 times in ox-LDL group. These results demonstrated that ox-LDL had different effect on the expression and function of ABCA1, ox-LDL might decrease the cholesterol efflux mediated by ABCA1 through other unknown mechanisms.
引用
收藏
页码：113 / 116
页数：3
相关论文
共 42 条
[1]  
Oram J F(2001)ABCA1: The gatekeeper for eliminating excess tissue cholesterol J Lipid Res 42 1173-1173
[2]  
Lawn R M(2001)Sterol efflux mediated by endogenous macrophage ApoE expression is independent of ABCA1 Arterioscler Thromb Vasc Biol 21 2019-2019
[3]  
Huang Z H(2000)Decreased cellular cholesterol efflux is a common cause of familial hypoal-phalipoproteinemia: role of the ABCA1 gene mutations Atherosclerosis 152 457-457
[4]  
Lin C Y(2000)Transport of lipids from golgi to plasma membrane is defective in tangier disease patients and Abc1-deficient mice Nat Genet 24 192-192
[5]  
Oram J F(2002)Increased ABCA1 activity protects against atherosclerosis J Clin Invest 110 35-35
[6]  
Mott S(2002)Increased Atherosclerosis in Hyperlipidemic Mice with Inactivation of ABCA1 in marophages Arterioscler Thromb Vasc Biol 22 630-630
[7]  
Yu L(1989)Beyond cholesterol. Modifications of low-density lipoprotein that increase its atherogenicity N Engl J Med 320 915-915
[8]  
Marcil M(1995)Rapid solation of large amount of plasma VLDL and LDL by a two step ultracentrifugation J Tongji Med Univ 15 198-198
[9]  
Orso E(1976)Degradation of cationized low density lipoprotein and regulation of cholesterol metabolism in homozygous familial hypercholesterolemia fibroblasts Proc Natl Acad Sci USA 73 3178-3178
[10]  
Broccardo C(1997)A role for macrophage scavenger receptors in atherosclerosis and susceptibility to infection Nature 20 292-292
12345