Click chemistry for targeted protein ubiquitylation and ubiquitin chain formation

被引:0
|
作者
Daniel Rösner
Tatjana Schneider
Daniel Schneider
Martin Scheffner
Andreas Marx
机构
[1] Konstanz Research School Chemical Biology,Department of Chemistry
[2] University of Konstanz,Department of Biology
[3] Konstanz Research School Chemical Biology,undefined
[4] University of Konstanz,undefined
来源
Nature Protocols | 2015年 / 10卷
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摘要
This protocol uses CuAAC click chemistry along with two different methods for expansion of the genetic code to assemble protein-protein conjugates for studying the post-translational modification of proteins by ubiquitin (ubiquitylation).
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页码:1594 / 1611
页数:17
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