Click chemistry for targeted protein ubiquitylation and ubiquitin chain formation

被引:0
|
作者
Daniel Rösner
Tatjana Schneider
Daniel Schneider
Martin Scheffner
Andreas Marx
机构
[1] Konstanz Research School Chemical Biology,Department of Chemistry
[2] University of Konstanz,Department of Biology
[3] Konstanz Research School Chemical Biology,undefined
[4] University of Konstanz,undefined
来源
Nature Protocols | 2015年 / 10卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
This protocol uses CuAAC click chemistry along with two different methods for expansion of the genetic code to assemble protein-protein conjugates for studying the post-translational modification of proteins by ubiquitin (ubiquitylation).
引用
收藏
页码:1594 / 1611
页数:17
相关论文
共 50 条
  • [1] Click chemistry for targeted protein ubiquitylation and ubiquitin chain formation
    Roesner, Daniel
    Schneider, Tatjana
    Schneider, Daniel
    Scheffner, Martin
    Marx, Andreas
    NATURE PROTOCOLS, 2015, 10 (10) : 1594 - 1611
  • [2] Improving bioorthogonal protein ubiquitylation by click reaction
    Schneider, Daniel
    Schneider, Tatjana
    Roesner, Daniel
    Scheffner, Martin
    Marx, Andreas
    BIOORGANIC & MEDICINAL CHEMISTRY, 2013, 21 (12) : 3430 - 3435
  • [3] Assembly of branched ubiquitin oligomers by click chemistry
    Zhao, Xiaohui
    Scheffner, Martin
    Marx, Andreas
    CHEMICAL COMMUNICATIONS, 2019, 55 (87) : 13093 - 13095
  • [4] Protein Iodination by Click Chemistry
    Dong, Shouliang
    Moroder, Luis
    Budisa, Nediljko
    CHEMBIOCHEM, 2009, 10 (07) : 1149 - 1151
  • [5] Mechanisms of Ubiquitin Chain Formation
    Wang Ya-Nan
    Zhao Bo
    PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2021, 48 (05) : 515 - 528
  • [6] Using Chemistry to Investigate the Molecular Consequences of Protein Ubiquitylation
    Abeywardana, Tharindumala
    Pratt, Matthew R.
    CHEMBIOCHEM, 2014, 15 (11) : 1547 - 1554
  • [7] A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein-Protein Conjugates
    Baalmann, Mathis
    Neises, Laura
    Bitsch, Sebastian
    Schneider, Hendrik
    Deweid, Lukas
    Werther, Philipp
    Ilkenhans, Nadja
    Wolfring, Martin
    Ziegler, Michael J.
    Wilhelm, Jonas
    Kolmar, Harald
    Wombacher, Richard
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2020, 59 (31) : 12885 - 12893
  • [8] A Pyrrolysine Analogue for Protein Click Chemistry
    Fekner, Tomasz
    Li, Xin
    Lee, Marianne M.
    Chan, Michael K.
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (09) : 1633 - 1635
  • [9] Reversible Click Chemistry for Ultrafast and Quantitative Formation of Protein-Polymer Nanoassembly and Intracellular Protein Delivery
    Liu, Bin
    Ianosi-Irimie, Margareta
    Thayumanavan, S.
    ACS NANO, 2019, 13 (08) : 9408 - 9420
  • [10] The self-assembled monolayer of saccharide via click chemistry: Formation and protein recognition
    Miura, Yoshiko
    Yamauchi, Takahiro
    Sato, Hajime
    Fukuda, Tomohiro
    THIN SOLID FILMS, 2008, 516 (09) : 2443 - 2449