Seroprevalence of porcine torovirus (PToV) in Spanish farms

被引：10

作者：

Alonso-Padilla J. ^{[1
,4
]}

Pignatelli J. ^{[2
]}

Simon-Grifé M. ^{[3
]}

Plazuelo S. ^{[1
]}

Casal J. ^{[3
]}

Rodríguez D. ^{[1
]}

机构：

[1] Department of Molecular and Cellular Biology, Centro Nacional de Biotecnología, CSIC, Madrid, 28049

[2] Department of Molecular, Cellular and Developmental Neurobiology, Instituto Cajal, CSIC, Madrid, 28002

[3] Centre de Recerca en Sanitat Animal (CReSA), Department of Animal Health, Universitat Autonoma de Barcelona, Bellaterra, Barcelona

[4] Department of Microbiology, New York University School of Medicine, NY 10010

来源：

BMC Research Notes | / 5卷 / 1期

关键词：

Pigs; Porcine torovirus; Seroprevalence; Spain;

D O I：

10.1186/1756-0500-5-675

中图分类号：

学科分类号：

摘要：

Background: Torovirus infections have been associated with gastroenteritis and diarrhea in horses, cows, pigs and humans, especially in young animals and in children. Although asymptomatic in a large percentage of cases, however toroviruses may pose a potential threat to worsen disease outcome in concurrent infections with other enteric pathogens. Previous studies based on the analysis of limited numbers of samples indicated high seroprevalences against porcine torovirus (PToV) in various European countries. The aim of this work was to perform a seroepidemiological survey of PToV in Spanish farms in order to define the seroprevalence against this virus. Results: Serum samples (n = 2664) from pigs of different ages were collected from 100 Spanish farms coming from 10 regions that concentrate 96.1% of the 3392 farms with 80 or more sows censused in Spain. Samples were screened by means of an indirect enzyme-linked immune-sorbent assay (ELISA) based on a recombinant PToV nucleocapsid protein as antigen. The analysis of the whole serum collection yielded a total of 95.7% (2550/2664) seropositive samples. The highest prevalence (99.6%, 1382/1388) and ELISA values (average O.D. ± standard deviation) were observed in the sows (1.03±0.36) and the lowest prevalence (59.4%, 98/165) and anti-PToV IgG levels (0.45±0.16) were found amongst 3-week-old piglets. Both ELISA reactivity values and seroprevalence percentages rose quickly with piglet's age from 3 to 11 weeks of age; the seroprevalence was 99.3% (2254/2270) when only the samples from sows and pigs over 11-weeks of age were considered. Antibodies against PToV were detected in all analyzed farms. Conclusions: This report describes the results of the largest torovirus seroepidemiological survey in farmed swine performed so far. Overall, the seroprevalence against PToV in animals older than 11 weeks of age was >99%, indicating that this virus is endemic in pig herds from Spain. © 2012 Alonso-Padilla et al.; licensee BioMed Central Ltd.

引用

共 23 条

[1]

Hoet A.E., Horzinek M.C., Torovirus, Encyclopedia of Virology (Third Edition), pp. 151-157, (2008)

[2]

De Vries A.A.F., Horzinek M.C., Rottier P.J.M., De Groot R.J., The genome organization of the Nidovirales: Similarities and differences between arteri-, toro-, and coronaviruses, Semin Virol, 8, pp. 33-47, (1997)

[3]

Weiss M., Steck F., Horzinek M.C., Purification and partial characterization of a new enveloped RNA virus (Berne virus), J Gen Virol, 64, pp. 1849-1858, (1983)

[4]

Woode G.N., Reed D.E., Runnels P.L., Herrig M.A., Hill H.T., Studies with an unclassified virus isolated from diarrheic calves, Vet Microbiology, 7, pp. 221-240, (1982)

[5]

Woode G.N., Breda and Breda-like viruses: Diagnosis, pathology and epidemiology, CIBA Symposium Series, pp. 175-191, (1987)

[6]

Woode G.N., Pohlenz J.F.L., Kelso-Gourley N.E., Fagerland J.A., Astrovirus and Breda virus infections of dome epihelium of bovine ileum, J Clin Microbiol, 19, 5, pp. 623-630, (1984)

[7]

Hoet A.E., Saif L.J., Bovine torovirus (Breda virus) revisited, Anim Health Res Rev, 5, 2, pp. 157-171, (2004)

[8]

Kuwabara M., Wada K., Maeda Y., Miyazaki A., Tsunemitsu H., First isolation of cytopathogenic bovine torovirus in cell culture from a calf with diarrhea, Clin Vaccine Immunol, 14, 8, pp. 998-1004, (2007)

[9]

Aita T., Kuwabara M., Murayama K., Sasagawa Y., Yabe S., Higuchi R., Tamura T., Miyazaki A., Tsunemitsu H., Characterization of epidemic diarrhea outbreaks associated with bovine torovirus in adult cows, Arch Virol, (2011)

[10]

Beards G.M., Brown D.W., Green J., Flewett T.H., Preliminary characterization of torovirus-like particles of humans: Comparison with the Berne virus of horses and the Breda virus of calves, J Med Virol, 20, pp. 67-78, (1986)

← 1 2 3 →