Molecular and cytogenic analysis of pericentromeric heterochromatin in ovarian nurse cells of Drosophila melanogaster subgroup species

被引：0

作者：

Usov K.E. ^{[1
]}

Shelkovnikova T.A. ^{[1
]}

Wasserlauf I.E. ^{[1
]}

Stegniy V.N. ^{[1
]}

机构：

[1] Research Institute of Biology and Biophysics, Tomsk State University, Tomsk

来源：

Cell and Tissue Biology | 2009年 / 3卷 / 1期

基金：

俄罗斯基础研究基金会;

关键词：

Drosophila melanogaster species subgroup; FISH analysis; Microdissection of chromosomes; Ovarian nurse cell polythene chromosomes; Pericentromeric heterochromatin;

D O I：

10.1134/S1990519X09010076

中图分类号：

学科分类号：

摘要：

Evolutionary rearrangements of pericentromeric heterochromatin among Drosophila melanogaster subgroup species have been investigated. A region-specific DNA library from Drosophila orena ovarian nurse cell chromocenter was obtained by the microdissection of polythene chromosomes. The probe has been localized on chromosomes of ovarian nurse cells of Drosophila melanogaster subgroup species using fluorescent hybridization in situ. Sequences homologous to the sequences of the DNA probe were detected in the chromocenter and pericentromeric regions of D. orena polythene chromosomes, in all pericentromeric regions of other species with several exceptions. There was no labeling on one of the arms of the D. simulans chromosome 2; however, these sequences were present on the telomere of D. erecta chromosome 3 and in regions adjacent to the brightly DAPI-stained heterochromatin blocks of D. yakuba, D. santomea and D. teissieri chromosomes 2 and 3. At the S6 stage (secondary reticulate nucleus), labeled chromatin can be found mostly within a restricted territory in D. orena nucleus; no such chromatin can be detected throughout the rest of the nucleus. On the contrary, at this stage, in nuclei of other species, labeled DNA is spread diffusely. © Pleiades Publishing, Ltd. 2009.

引用

页码：50 / 55

页数：5

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