A novel compound JPM8: in vivo penile activity promotion in rats, effect on the relaxation and cGMP/cAMP accumulation in isolated rabbit corpora cavernosa

被引：0
作者：
T S El-Thaher
S Khatib
M Saleem
A Shnoudeh
A A Badwan
机构：
[1] The Jordanian Pharmaceutical Manufacturing Ltd,Physiology Department
[2] Faculty of Medicine,Department of Pharmacy
[3] Jordan University of Science and Technology,undefined
[4] School of Health Sciences,undefined
[5] King′s College,undefined
[6] University of London,undefined
来源：
International Journal of Impotence Research | 2002年 / 14卷
关键词：
JPM8; phosphodiesterase type 5 (PDE 5); inhibitors; erectile dysfunction; sildenafil; cGMP;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
JPM8 is a novel sildenafil-like PDE5 inhibitor. Its efficacy was tested in vivo by the oral administration of drugs to a rat model and recording penile activity changes. Effect on the relaxation of the rabbit cavernosa was tested in vitro using an organ bath were drugs are added to the tissue media and relaxation was recorded using a transducer connected to a chart recorder. The accumulation of cGMP and cAMP was measured by incubation of cavernosa strips and then extracting the produced cGMP and cAMP in the incubation mixture, then quantitating it using ELISA. JPM8 showed increased and promoted sexual and penile activity in rats in a similar but slightly higher trend than the positive control sildenafil. JPM8 was more efficient in relaxing the rabbit corpora cavernosa than sildenafil. The cGMP and cAMP accumulation showed a similar trend for both drugs. We concluded that JPM8 was very effective in promoting sexual activity in rats, relaxing the corpora cavernosa and promoting cGMP accumulation in rabbits.
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页码：453 / 461
页数：8
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