Correlation of Peroxisome Proliferator-Activated Receptor (PPAR-γ) mRNA Expression with Pro12Ala Polymorphism in Obesity

被引：0
作者：
Rym Berhouma
Soumaya Kouidhi
Myriam Ammar
Hafawa Abid
Hajer Ennafaa
Amel Benammar-Elgaaied
机构：
[1] University of El Manar 1,Laboratory of Genetics, Immunology and Human Pathologies, Faculty of Sciences of Tunis
[2] Hospital of Charles Nicolle,undefined
来源：
Biochemical Genetics | 2013年 / 51卷
关键词：
Obesity; expression; Pro12Ala polymorphism; Adipocytes;
D O I：
暂无
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学科分类号：
摘要：
Our study aimed to analyze whether the expression of PPARγ mRNA in subcutaneous adipocyte tissue correlates with Pro12Ala PPARγ2 polymorphism in the obesity context. We found that mRNA expression of PPARγ in subcutaneous adipose tissue was greater in obese subjects (P < 0.05) than in the nonobese control group. Concurrently, genotyping of the Pro12Ala polymorphism showed that obese subjects possess a significantly higher frequency of the Pro/Pro genotype than nonobese controls (90.5 vs 79.5%; P = 0.03), suggesting that this genotype is involved in an increased risk of obesity in the Tunisian population. Taken together, our results demonstrate that the Pro12 allele is accompanied by an overexpression of PPARγ mRNA in subcutaneous adipocyte tissue, suggesting that the PPARγ Pro12Ala variant may contribute to the observed variability in PPARγ mRNA expression and consequently in body mass index and insulin sensitivity in the general population.
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页码：256 / 263
页数：7
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共 202 条
[1]  
Altshuler D(2000)The common PPARγ Pro12Ala polymorphism is associated with decreased risk of type 2 diabetes Nat Genet 26 76-80
[2]  
Hirschhorn JN(2004)Diagnosis and classification of diabetes mellitus Diabetes Care 27 S5-S10
[3]  
Klannemark M(2007)Fat poetry: a kingdom for PPAR γ Cell Res 17 486-511
[4]  
Lindgren CM(1999)PPARγ is required for placental, cardiac, and adipose tissue development Mol Cell 4 585-595
[5]  
Vohl MC(1998)Association of the Pro12Ala variant in the peroxisome proliferator-activated receptor γ2 gene with obesity in two Caucasian populations Diabetes 47 1806-1808
[6]  
Nemesh J(2009)Gender-specific effect of Pro12Ala polymorphism in peroxisome proliferator-activated receptor γ-2 gene on obesity risk and leptin levels in a Tunisian population Clin Biochem 42 1642-1647
[7]  
Lane CR(2008)Effect of ENPP1/PC-1-K121Q and PPARγ-Pro12Ala polymorphisms on the genetic susceptibility to type 2 diabetes in the Tunisian population Diabetes Res Clin Pract 81 278-283
[8]  
Schaffner SF(2000)The Pro115Gln and Pro12Ala PPAR γ gene mutations in obesity and type 2 diabetes Int J Obes Relat Metab Disord 24 391-393
[9]  
Bolk S(2011)Forming functional fat: a growing understanding of adipocyte differentiation Nat Rev Mol Cell Biol 12 722-734
[10]  
Brewer C(1998)A Pro12Ala substitution in PPARγ2 associated with decreased receptor activity, lower body mass index and improved insulin sensitivity Nat Genet 20 284-287
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