Effects of aqueous leaf extracts of Loranthus micranthus Linn. on hematological profile of albino rats infected with Trypanosoma brucei brucei

被引：0

作者：

Egbuji J.V. ^{[1
]}

Ejere V.C. ^{[1
]}

Ugwu G.C. ^{[1
]}

Okanya C.L. ^{[1
]}

机构：

[1] Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State

来源：

Comparative Clinical Pathology | 2019年 / 28卷 / 5期

关键词：

Albino rats; Aqueous leaf extracts; Hematological profile; Loranthus micranthus; Parasitaemia; Trypanosomiasis;

D O I：

10.1007/s00580-019-02973-4

中图分类号：

学科分类号：

摘要：

African trypanosomiasis has continued to threaten human health and economical development (Kuzoe Acta Trop 54:153–162, 1993; WHO J Tsetse Tryp Info Quart 3:4–9, 2000). It is caused by parasitic protozoa of the genus Trypanosoma (Adeiza et al. J Med Plant Res 4(17):1770–1777, 2010), and the distribution corresponds with that of tsetse flies. Effects of aqueous leaf extracts of Loranthus micranthus on hematological parameters of albino rats infected with Trypanosoma brucei brucei were investigated for 28 days using standard methods. The HB, PCV, RBC, and its indices (MCH, MCV, and MCHC) of the infected rats significantly decreased (P < 0.05) at various dose levels of the extracts when compared with the control groups. The WBC counts of the treatment groups and those of the negative control group showed significant increases (P < 0.05) in all the weeks when compared with the normal control. The WBC differentials revealed that neutrophils were significantly higher (P < 0.05) in the test group in comparison with the positive control group at week 3; however, lymphocytes, eosinophils, and basophils were not significantly different (P > 0.05) from the positive control in week 3. Furthermore, minimal increases in the WBC differentials were observed in the group administered 800 mg/kg of the plant extract. The present study showed that all test rats and the negative control group died from the resultant overwhelming parasitemia at week 4 unlike the case of those administered the standard drug, which is an indication that the extract lacks anti-trypanocidal activity. Thus, the aqueous leaf extract of Loranthus micranthus is an inadequate anti-trypanosomal agent. © 2019, Springer-Verlag London Ltd., part of Springer Nature.

引用

页码：1373 / 1380

页数：7

共 44 条

[1]

Abubakar A., Iliyasu B., Yusuf A.B., Igweh A.C., Onyekwelu N.A., Shamaki B.A., Afolayan D.O., Ogbadoyi E.O., Antitrypanosomal and haematological effects of selected Nigerian medicinal plants in Wistar rats, Biokemistri, 17, pp. 95-99, (2005)

[2]

Adeiza A.A., Mohammed A., Mamman M., Comparative in vivo evaluation of the trypanocidal activities of aqueous leaf, stem-bark, and root extracts of Khaya senegalensis on Trypanosoma evansi, J Med Plant Res, 4, 17, pp. 1770-1777, (2010)

[3]

Adeyemi O.S., Akanji M.A., Oguntoye S.A., Ethanolic leaf extract of Psidium guajava: Phyto-chemical and trypanocidal activity in rats infected with Trypanosome brucei brucei, J Med Plant Res, 3, 5, pp. 420-423, (2009)

[4]

Aliyu S.M., Shetty S.N., Asuzu I.U., Chime A.B., Effects of some trypanosides and inflammatory agents in experimental Trypanosoma brucei in mice, Zariya Vet J, 2, pp. 9-15, (2010)

[5]

Anosa V.O., Kaneko J.J., Pathogenesis of Trypanosoma brucei infection in deer mice (Peromyscus maniculatus): hematologic, erythrocyte, biochemical and iron metabolic aspects, Am Vet Res, 44, 4, pp. 639-644, (1983)

[6]

Aroke A.H., Asonganyi T., Mbonda E., Influence of a past history of Gambian sleeping sickness on physical growth, sexual maturity and academic performance of children in Fontem, Cameroon, Ann Trop Med Parasitol, 92, pp. 829-835, (1998)

[7]

Ashafa A.O., Yakubu M.T., Grierson D.S., Afolayan A.J., Effects of aqueous leaf extract from the leaves of Chrysocoma ciliate L. on some biochemical parameters of Wistar rats, Afr J Biotechnol, 8, pp. 1425-1430, (2009)

[8]

Bakers F.J., Silverton R.E., Pallister C.J., Introduction to medical laboratory technology, (2001)

[9]

Barry J.D., Turner C.M., The dynamic of antigenic variation and growth of African trypanosomes, Parasitol Today, 7, pp. 207-211, (1992)

[10]

Burri C., Nlimli S., Merolle A., Smith T., Brun R., Efficacy of new concise schedule for melarsoprol in treatment of sleeping sickness caused by Trypansosoma brucei gambiense: a randomized trial, Lancet, 355, 9213, pp. 1419-1425, (2000)

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