The cytotoxic constituents from marine-derived streptomyces 3320#

被引:0
|
作者
Ren Hong
Gu Qianqun
Cui Chengbin
Zhu Weiming
机构
[1] Ocean University of China,Key Laboratory of Marine Drug Chinese Ministry of Education, Institute of Marine Drug and Food
[2] Yantai University,Science and Technology College of Chemistry and Biology
[3] AMMS,Beijing Institute of Pharmacology and Toxicology
关键词
marine-derived streptomyces; secondary metabolites; structural identify; bioassay-guided fractionation; antitumor activity;
D O I
10.1007/BF02919379
中图分类号
学科分类号
摘要
The present work studies the chemical constituents from marine-derived streptomyces 3320# and their antitumor activities. Then-BuOH extract of the ferment broth of 3320# was chromatographed on silica gel, Sephadex LH-20, ODS columns and HPLC to separate the compounds with antitoumor activities. Their structures were identified using IR, UV, NMR, MS spectroscopic techniques and compared with published data. The antitumor activities of the isolates were assayed using SRB method and flow cytometry assay, accompanied with the morphological observation of the cells under light microscope against mammalian tsFT210 cells. Ten compounds, cyclo-(Ala-Leu) 1, cyclo-(Ala-Ile) 2, cyclo-(Ala-Val) 3, cyclo-(Phe- Pro) 4, cyclo-(Phe-Gly) 5, cyclo-(Leu-Pro) 6, 1-methyl-1, 2, 3, 4-tetrahydro-β-carboline-3-carboxylic acid 7, N-(4-hydroxyphenethyl) acetamide 8, 4-methyoxy-1-(2-hydroxy) ethylbenzene 9 and uridine 10, were isolated from the ferment broth of streptomyces 3320#. Among them, compounds 6, 7, 8 and 10 showed potent cytotoxicity against the tsFT210 cell with the IC50 values of 3.6, 7.2, 5.2 and 1.6 mmol L−1, respectively. Compounds 8, 10 also exhibited apoptosis inducing activity under 2.0 mmol L−1. Compounds 6, 7, 8 and 10 are the principle bioactive constituents responsible for the antitumor activities of marine streptomyces 3320#. Compound 7 was isolated from this species for the first time.
引用
收藏
页码:75 / 81
页数:6
相关论文
共 50 条
  • [21] Octyl substituted butenolides from marine-derived Streptomyces koyangensis
    Huang, Hongbo
    Song, Yongxiang
    Zang, Ruochen
    Wang, Xin
    Ju, Jianhua
    NATURAL PRODUCT RESEARCH, 2021, 35 (15) : 2602 - 2607
  • [22] Chalcomycins from Marine-Derived Streptomyces sp and Their Antimicrobial Activities
    Jiang, Shutai
    Zhang, Lili
    Pei, Xuechang
    Deng, Fang
    Hu, Dan
    Chen, Guodong
    Wang, Chuanxi
    Hong, Kui
    Yao, Xinsheng
    Gao, Hao
    MARINE DRUGS, 2017, 15 (06):
  • [23] Antibacterial Activity of Chromomycins from a Marine-Derived Streptomyces microflavus
    Cho, Eunji
    Kwon, Oh-Seok
    Chung, Beomkoo
    Lee, Jayho
    Sun, Jeongyoon
    Shin, Jongheon
    Oh, Ki-Bong
    MARINE DRUGS, 2020, 18 (10)
  • [24] Neuraminidase Inhibitors from marine-derived actinomycete Streptomyces seoulensis
    Jiao, R. H.
    Xu, H.
    Cui, J. T.
    Ge, H. M.
    Tan, R. X.
    JOURNAL OF APPLIED MICROBIOLOGY, 2013, 114 (04) : 1046 - 1053
  • [25] Streptokordin, a new cytotoxic compound of the methylpyridine class from a marine-derived Streptomyces sp KORDI-3238
    Jeong, Seong-Yun
    Shin, Hee Jae
    Kim, Tae Sik
    Lee, Hyi-Seung
    Park, Song-kyu
    Kim, Hwan Mook
    JOURNAL OF ANTIBIOTICS, 2006, 59 (04): : 234 - 240
  • [26] Cytotoxic bafilomycin analogues 6/5/5 with tricyclic ring system from a marine-derived Streptomyces sp
    Li, Jia-Qi
    Zhao, Hao-Wen
    Ma, Zhong-Jun
    TETRAHEDRON LETTERS, 2020, 61 (21)
  • [27] Cytotoxic and Antifungal Staurosporine Derivatives from Marine-Derived Actinomycete Streptomyces sp. ZS-A121
    Xu, Lei
    Liu, Renshuang
    Fang, Jiebin
    Zhang, Ningjing
    Pu, Fanqi
    Lei, Zhen
    Ding, Wanjing
    Jiang, Yongjun
    CHEMISTRY & BIODIVERSITY, 2024, 21 (02)
  • [28] Cytotoxic Cytochalasins from Marine-Derived Fungus Arthrinium arundinis
    Wang, Junfeng
    Wang, Zhen
    Ju, Zhiran
    Wan, Junting
    Liao, Shengrong
    Lin, Xiuping
    Zhang, Tianyu
    Zhou, Xuefeng
    Chen, Hao
    Tu, Zhengchao
    Liu, Yonghong
    PLANTA MEDICA, 2015, 81 (02) : 160 - 166
  • [29] Marineosins A and B, Cytotoxic Spiroaminals from a Marine-Derived Actinomycete
    Boonlarppradab, Chollaratt
    Kauffman, Christopher A.
    Jensen, Paul R.
    Fenical, William
    ORGANIC LETTERS, 2008, 10 (24) : 5505 - 5508
  • [30] Cytotoxic Polyphenols from the Marine-Derived Fungus Penicillium expansum
    Lu, Zhenyu
    Zhu, Huajie
    Fu, Peng
    Wang, Yi
    Zhang, Zhihua
    Lin, Haipeng
    Liu, Peipei
    Zhuang, Yibin
    Hong, Kui
    Zhu, Weiming
    JOURNAL OF NATURAL PRODUCTS, 2010, 73 (05): : 911 - 914