The cytotoxic constituents from marine-derived streptomyces 3320#

被引:0
|
作者
Ren Hong
Gu Qianqun
Cui Chengbin
Zhu Weiming
机构
[1] Ocean University of China,Key Laboratory of Marine Drug Chinese Ministry of Education, Institute of Marine Drug and Food
[2] Yantai University,Science and Technology College of Chemistry and Biology
[3] AMMS,Beijing Institute of Pharmacology and Toxicology
关键词
marine-derived streptomyces; secondary metabolites; structural identify; bioassay-guided fractionation; antitumor activity;
D O I
10.1007/BF02919379
中图分类号
学科分类号
摘要
The present work studies the chemical constituents from marine-derived streptomyces 3320# and their antitumor activities. Then-BuOH extract of the ferment broth of 3320# was chromatographed on silica gel, Sephadex LH-20, ODS columns and HPLC to separate the compounds with antitoumor activities. Their structures were identified using IR, UV, NMR, MS spectroscopic techniques and compared with published data. The antitumor activities of the isolates were assayed using SRB method and flow cytometry assay, accompanied with the morphological observation of the cells under light microscope against mammalian tsFT210 cells. Ten compounds, cyclo-(Ala-Leu) 1, cyclo-(Ala-Ile) 2, cyclo-(Ala-Val) 3, cyclo-(Phe- Pro) 4, cyclo-(Phe-Gly) 5, cyclo-(Leu-Pro) 6, 1-methyl-1, 2, 3, 4-tetrahydro-β-carboline-3-carboxylic acid 7, N-(4-hydroxyphenethyl) acetamide 8, 4-methyoxy-1-(2-hydroxy) ethylbenzene 9 and uridine 10, were isolated from the ferment broth of streptomyces 3320#. Among them, compounds 6, 7, 8 and 10 showed potent cytotoxicity against the tsFT210 cell with the IC50 values of 3.6, 7.2, 5.2 and 1.6 mmol L−1, respectively. Compounds 8, 10 also exhibited apoptosis inducing activity under 2.0 mmol L−1. Compounds 6, 7, 8 and 10 are the principle bioactive constituents responsible for the antitumor activities of marine streptomyces 3320#. Compound 7 was isolated from this species for the first time.
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页码:75 / 81
页数:6
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