Efficient ex-vitro rooting and acclimatization for tissue culture plantlets of ginger

被引:0
作者
Jie Zhou
Fengling Guo
Chuangdong Qi
Jiaping Fu
Ying Xiao
Jinping Wu
机构
[1] Hubei Academy of Agricultural Sciences,Institute of Economic Crops
[2] Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement,undefined
来源
Plant Cell, Tissue and Organ Culture (PCTOC) | 2022年 / 150卷
关键词
Ginger; Micropropagation; Ex-vitro rooting; Acclimatization;
D O I
暂无
中图分类号
学科分类号
摘要
Ginger (Zingiber officinale), a member of the Zingiberaceae family, is a commonly available spice and medicinal plant. Ginger plantlets in tissue culture have not been commercialized because their ultimate success depends on the ability to successfully transfer the tissue culture plantlets from a controlled, aseptic environment to farm land while maintaining low costs and high survival rates. The survival rate of tissue culture plantlets transferred from a lab to soil is low, mainly because of differences in temperature, humidity conditions, biotic stresses, etc., which makes obtaining new seedlings challenging. This research aimed to optimize the protocol for acclimatization of tissue culture ginger plantlets. Rootless ginger tissue culture plantlets were initiated in seedling trays grown in a chamber maintained under a 16 h/day photoperiod with 60 μE m−2 s−1 of cool white light from fluorescent lamps at 25 ± 2 °C with an air relative humidity of 60 ± 5%, and the optimum substrate was vermiculite + peat (1:1 v/v) with MS + 0.5 mg/L NAA + 0.1% Metalaxyl-M·Hymexazol + 80% relative humidity (RH). After 40 days of culture, ex-vitro ginger seedlings had been successfully bred with a rooting rate of 100.0%, and the seedlings could be directly transplanted to the field.
引用
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页码:451 / 458
页数:7
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