Effects In Vitro of Guanidinoacetate on Adenine Nucleotide Hydrolysis and Acetylcholinesterase Activity in Tissues from Adult Rats

被引：0
作者：
Roselia Maria Spanevello
Angela Terezinha de Souza Wyse
Cinthia Melazzo Mazzanti
Roberta Schmatz
Naiara Stefanello
Jamile Fabbrin Gonçalves
Margarete Bagatini
Vanessa Battisti
Vera Maria Morsch
Maria Rosa Chitolina Schetinger
机构：
[1] Universidade Federal do Rio Grande do Sul,Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde
[2] Universidade Federal de Santa Maria,Departamento de Química, Centro de Ciências Naturais e Exatas
来源：
Neurochemical Research | 2008年 / 33卷
关键词：
Guanidinoacetate; NTPDase; 5′-nucleotidase; Acetylcholinesterase; Synaptosomes; Platelets;
D O I：
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中图分类号：
学科分类号：
摘要：
Guanidinoacetate methyltransferase (GAMT) deficiency is a disorder of creatine metabolism characterized by low plasma creatine concentrations in combination with elevated guanidinoacetate (GAA) concentrations. The aim of this work was to investigate the in vitro effect of guanidinoacetate in NTPDase, 5′-nucleotidase and acetylcholinesterase activities in the synaptosomes, platelets and blood of rats. The results showed that in synaptosomes the NTPDase and 5′-nucleotidase activities were inhibited significantly in the presence of GAA at concentrations of 50, 100, 150 and 200 μM (P < 0.05). However, in platelets GAA at the same concentrations caused a significant increase in the activities of these two enzymes (P < 0.05). In relation to the acetylcholinesterase activity, GAA caused a significant inhibition in the activity of this enzyme in blood at concentrations of 150 and 200 μM (P < 0.05), but did not alter the acetylcholinesterase activity in synaptosomes from the cerebral cortex. Our results suggest that alterations caused by GAA in the activities of these enzymes may contribute to the understanding of the neurological dysfunction of GAMT-deficient patients.
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页码：1129 / 1137
页数：8
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