Transcriptional response of human mast cells stimulated via the Fcε RI and identification of mast cells as a source of IL-11

被引：48

作者：

Sayama K. ^{[1
]}

Diehn M. ^{[2
]}

Matsuda K. ^{[1
]}

Lunderius C. ^{[1
]}

Tsai M. ^{[1
]}

Tam S.-Y. ^{[1
]}

Botstein D. ^{[3
]}

Brown P.O. ^{[2
,4
]}

Galli S.J. ^{[1
]}

机构：

[1] Department of Pathology, Stanford Univ. School of Medicine, Stanford, CA

[2] Department of Biochemistry, Stanford Univ. School of Medicine, Stanford, CA

[3] Department of Genetics, Stanford Univ. School of Medicine, Stanford, CA

[4] Howard Hughes Medical Institute, Stanford Univ. School of Medicine, Stanford, CA

来源：

BMC Immunology | / 3卷 / 1期

关键词：

Mast Cell; Tace; Allergic Asthma; Transcriptional Program; Human Mast Cell;

D O I：

10.1186/1471-2172-3-5

中图分类号：

学科分类号：

摘要：

Background: In asthma and other allergic disorders, the activation of mast cells by IgE and antigen induces the cells to release histamine and other mediators of inflammation, as well as to produce certain cytokines and chemokines. To search for new mast cell products, we used complementary DNA microarrays to analyze gene expression in human umbilical cord blood-derived mast cells stimulated via the high-affinity IgE receptor (FcERI). Results: One to two hours after FcERI-dependent stimulation, more than 2,400 genes (about half of which are of unknown function) exhibited 2-200 fold changes in expression. The transcriptional program included changes in the expression of IL-11 and at least 30 other cytokines and chemokines. Human mast cells secreted 130-529 pg of IL-11/106 cells by 6 h after stimulation with anti-IgE. Conclusion: Our initial analysis of the transcriptional program induced in in vitro-derived human mast cells stimulated via the FcERI has identified many products that heretofore have not been associated with this cell type, but which may significantly influence mast cell function in IgE-associated host responses. We also have demonstrated that mast cells stimulated via the FcERI can secrete IL-11. Based on the previously reported biological effects of IL-11, our results suggest that production of IL-11 may represent one link between IgE-dependent mast cell activation in subjects with allergic asthma and the development of a spectrum of structural changes in the airways of these individuals; such changes, collectively termed "airway remodeling," can constitute an important long term consequence of asthma. © 2002 Sayama et al; licensee BioMed Central Ltd.

引用

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