Characterization, solubility and antibacterial activity of inclusion complex of questin with hydroxypropyl-β-cyclodextrin

被引：0
作者：
Lei Guo
Xi Cao
Shulin Yang
Xintong Wang
Ying Wen
Fei Zhang
Hui Chen
Le Wang
机构：
[1] Huaihai Institute of Technology,Jiangsu Key Laboratory of Marine Bioresources and Environment
[2] Huaihai Institute of Technology,Jiangsu Key Laboratory of Marine Biotechnology, Co
[3] Jiangsu Institute of Marine Resources Development,innovation Center of Jiangsu Marine Bio
来源：
3 Biotech | 2019年 / 9卷
关键词：
Questin; 2-Hydroxypropyl-β-cyclodextrin; Inclusion complex; Solubility; Antibacterial activity;
D O I：
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中图分类号：
学科分类号：
摘要：
To increase the water solubility of questin and broaden its application in preventing and treating Vibrio diseases in aquaculture, an inclusion complex of questin with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) was prepared by stirring and coevaporation strategy. The results of thin-layer chromatography and nuclear magnetic resonance spectrum confirmed the inclusion of questin into HP-β-CD. The aqueous solubility of questin in the inclusion complex reached (62.63 ± 1.21) µg/mL, which was 110 times of questin’s original solubility. The preliminary agar diffusion method indicated that questin–HP-β-CD inclusion complex showed enhanced antibacterial activity against Vibrio harveyi compared with free questin. This finding provided a reliable basis for the further application of questin as aquatic antibacterial agent.
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