Effect of short hairpin RNA of Bcl-xL gene on biological behaviors of HepG-2 cells

被引:0
作者
Duan Mengxi
Yuan Hong
Wang Nan
Li Shijun
Jiang Yanmei
机构
[1] The First Affiliated Hospital of Dalian Medical University,Department of Clinical Laboratory
来源
Medical Oncology | 2012年 / 29卷
关键词
RNA interference; Bcl-xL; Hepatocellular carcinoma;
D O I
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学科分类号
摘要
To investigate the inhibitory effect of short hairpin RNA (shRNA) targeting the Bcl-xL gene on the apoptosis and proliferation of hepatocellular carcinoma HepG-2 cells. We constructed Bcl-xL shRNA, and transfected it into HepG-2 cells, then detected Bcl-xL gene expression on mRNA and protein levels by reverse transcription–polymerase chain reaction (RT–PCR) and flow cytometry, respectively. Cell proliferation was detected by MTT assay. Cell apoptosis and Bcl-2 and Bax expression were detected by flow cytometry. The expression of Bcl-xL was obviously inhibited by RNA interference. The inhibition rates of Bcl-xL mRNA and protein were 86.6 and 70.2%, respectively. Bcl-xL shRNA inhibited cell proliferation (P < 0.05), whereas promoted apoptosis (P < 0.01). After transfection of Bcl-xL shRNA in HepG-2 cells, the expression of Bcl-2 did not change, but Bax increased significantly. Bcl-xL shRNA effectively inhibits Bcl-xL gene expression and proliferation of HepG-2 cells and promotes apoptosis.
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页码:1000 / 1004
页数:4
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