Expression, purification and characterization of the sulfite reductase hemo-subunit, SiR-HP, from Acidithiobacillus ferrooxidans

被引：0
作者：
Jia Zeng
Ming Wang
Xiaojian Zhang
Yiping Wang
Chenbin Ai
Jianshe Liu
Guanzhou Qiu
机构：
[1] Central South University,Department of Bioengineering, School of Resources Processing and Bioengineering
来源：
Biotechnology Letters | 2008年 / 30卷
关键词：
Expression; Mutation; Purification; Sulfite reductase;
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摘要：
Sulfite reductase (SiR) is a large and soluble enzyme which catalyzes the transfer of six electrons from NADPH to sulfite to produce sulfide. The sulfite reductase flavoprotein (SiR-FP) contains both FAD and FMN, and the sulfite reductase hemoprotein (SiR-HP) contains an iron–sulfur cluster coupled to a siroheme. The enzyme is arranged so that the redox cofactors in the FAD-FMN-Fe4S4−Heme sequence make an electron pathway between NADPH and sulfite. Here we report the cloning, expression, and characterization of the SiR-HP of the sulfite reductase from Acidithiobacillus ferrooxidans. The purified SiR-HP contained a [Fe4S4] cluster. Site-directed mutagenesis results revealed that Cys427, Cys433, Cys472 and Cys476 were in ligating with the [Fe4S4] cluster of the protein.
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页码：1239 / 1244
页数：5
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