Single-molecule force spectroscopy: Optical tweezers, magnetic tweezers and atomic force microscopy

被引：0
|
作者：
Neuman K.C. [1 ]
Nagy A. [1 ]
机构：
[1] Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892
来源：
Nature Methods | 2008年 / 5卷 / 6期
关键词：
D O I：
10.1038/nmeth.1218
中图分类号：
学科分类号：
摘要：
Single-molecule force spectroscopy has emerged as a powerful tool to investigate the forces and motions associated with biological molecules and enzymatic activity. The most common force spectroscopy techniques are optical tweezers, magnetic tweezers and atomic force microscopy. Here we describe these techniques and illustrate them with examples highlighting current capabilities and limitations.
引用
收藏
页码：491 / 505
页数：14
相关论文
共 50 条
  • [1] Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy
    Neuman, Keir C.
    Nagy, Attila
    NATURE METHODS, 2008, 5 (06) : 491 - 505
  • [2] Towards a new modular polyprotein system compatible with single-molecule force spectroscopy by atomic force microscopy and magnetic tweezers
    Velazquez-Carreras, Diana
    Gavilan-Herrera, Manuel
    Martinez-Martin, Ines
    Suay-Corredera, Carmen
    Dumitru, Andra C.
    Galan, Elias Herrero
    Alegre-Cebollada, Jorge
    BIOPHYSICAL JOURNAL, 2023, 122 (03) : 304A - 304A
  • [3] Dynamic single-molecule force spectroscopy using optical tweezers and nanopores
    Laohakunakorn, Nadanai
    Otto, Oliver
    Sturm, Sebastian
    Kroy, Klaus
    Keyser, Ulrich F.
    OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION X, 2013, 8810
  • [4] Single-Molecule FRET Combined with Magnetic Tweezers at Low Force Regime
    Uhm, Heesoo
    Bae, Sangsu
    Lee, Mina
    Hohng, Sungchul
    BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2016, 37 (03) : 408 - 410
  • [5] Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers
    Adhikari, Arjun S.
    Chai, Jack
    Dunn, Alexander R.
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2012, (65):
  • [6] An Optical Tweezers Platform for Single Molecule Force Spectroscopy in Organic Solvents
    Black, Jacob W.
    Kamenetska, Maria
    Ganim, Ziad
    NANO LETTERS, 2017, 17 (11) : 6598 - 6605
  • [7] Optical tweezers for single molecule force spectroscopy on bacterial adhesion organelles
    Andersson, Magnus
    Axner, Ove
    Uhlin, Bernt Enc
    Fallman, Erik
    OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION III, 2006, 6326
  • [8] Single-Molecule Force Spectroscopy Using the NanoTracker™ Optical Tweezers Platform: from Design to Application
    Wozniak, A.
    van Mameren, J.
    Ragona, S.
    CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2009, 10 (05) : 467 - 473
  • [9] NanoTracker: force-sensing optical tweezers for quantitative single-molecule nanomanipulation
    van Mameren, Joost
    Eggert, Helge
    Behme, Gerd
    Jaehnke, Torsten
    Neicke, Thomas
    EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2011, 40 : 215 - 215
  • [10] NanoTracker: Force-Sensing Optical Tweezers for Quantitative Single-Molecule Nanomanipulation
    Eggert, Helge A.
    van Mameren, Joost
    Wozniak, Anna
    Jaehnke, Torsten
    COMPLEX LIGHT AND OPTICAL FORCES IV, 2010, 7613
12345