Identification of resistance gene analogs linked to a powdery mildew resistance locus in grapevine

被引：0

作者：

T. M. Donald

F. Pellerone

A.-F. Adam-Blondon

A. Bouquet

M. R. Thomas

I. B. Dry

机构：

[1] CSIRO Plant Industry,

[2] Horticulture Unit,undefined

[3] PO Box 350,undefined

[4] Glen Osmond SA 5064,undefined

[5] Australia e-mail: ian.dry@pi.csiro.au Tel.: +61-883038600,undefined

[6] Fax: +61-883038601,undefined

[7] Present address: F. Pellerone,undefined

[8] Division of Biochemistry and Molecular Biology,undefined

[9] Faculty of Science,undefined

[10] Australian National University,undefined

[11] Canberra ACT 2600,undefined

[12] Australia,undefined

[13] UR-GAP Viticulture,undefined

[14] ENSAM-INRA,undefined

[15] 2 place P. Viala,undefined

[16] 34060 Montpellier cedex 1,undefined

[17] France,undefined

来源：

Theoretical and Applied Genetics | 2002年 / 104卷

关键词：

Keywords Nucleotide binding site (NBS); Leucine rich repeat (LRR); Uncinula necator; Vitis vinifera; Disease resistance; Resistance gene analog (RGA);

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Oligonucleotide primers, designed to conserved regions of nucleotide binding site (NBS) motifs within previously cloned pathogen resistance genes, were used to amplify resistance gene analogs (RGAs) from grapevine. Twenty eight unique grapevine RGA sequences were identified and subdivided into 22 groups on the basis of nucleic acid sequence-identity of approximately 70% or greater. Representatives from each group were used in a bulked segregant analysis strategy to screen for restriction fragment length polymorphisms linked to the powdery mildew resistance locus, Run1, introgressed into Vitis vinifera L. from the wild grape species Muscadinia rotundifolia. Three RGA markers were found to be tightly linked to the Run1 locus. Of these markers, two (GLP1–12 and MHD145) cosegregated with the resistance phenotype in 167 progeny tested, whereas the third marker (MHD98) was mapped to a position 2.4 cM from the Run1 locus. The results demonstrate the usefulness of RGA sequences, when used in combination with bulked segregant analysis, to rapidly generate markers tightly linked to resistance loci in crop species.

引用

页码：610 / 618

页数：8