Characterization of Citrus tristeza virus isolates in northern Iran

被引：8

作者：

Barzegar A. ^{[1
,2
]}

Sohi H.H. ^{[2
]}

Rahimian H. ^{[3
]}

机构：

[1] Department of Biology, Faculty of Science, Razi University, Kermanshah

[2] Plant Biotechnology Department, National Institute of Genetic Engineering and Biotechnology, Tehran, 15 S. S. A., G. St., Enghelab Ave.

[3] College of Agriculture, Mazandaran University, Sari, Khazarabad Street

来源：

Journal of General Plant Pathology | 2006年 / 72卷 / 1期

关键词：

Biological properties; Citrus tristeza virus; Coat protein gene; Iranian isolates; Nucleotide sequencing; RFLP;

D O I：

10.1007/s10327-005-0249-5

中图分类号：

学科分类号：

摘要：

The biological and molecular properties of four Citrus tristeza virus (CTV) isolates isolated from infected Satsuma trees imported from Japan, and growing in citrus groves in northern Iran (Mahdasht orchards, Mazandaran Province), were investigated. CTV-infected samples were collected from sweet orange trees and grafted onto Alemow (Citrus macrophylla Wester) seedlings. On indicator plants, these isolates produced various symptoms including vein clearing and stem pitting on Mexican lime, Alemow, and Citrus hystrix, and yellowing and stunting on sour orange and grapefruit seedlings. Citrus samples were also surveyed for CTV using serological tests. The coat protein (CP) gene of these isolates was amplified using specific primers, yielding an amplicon of 672 bp for all isolates. Sequence analysis showed 98%-99% sequence homology of Iranian isolates with the Californian CTV severe stem-pitting isolate SY568 and 97%-98% homology with the Japanese seedling yellows isolate NUagA. The Iranian isolates were compared by restriction fragment length polymorphism (RFLP) analysis of the CP amplicon for further classification. © The Phytopathological Society of Japan and Springer-Verlag 2006.

引用

页码：46 / 51

页数：5

共 32 条

[1]

Albiach-Marti M.R., Guerri J., Hermoso De Mendoza A., Laigret F., Ballester-Olmos J.F., Moreno P., Aphid transmission alters the genomic and defective RNA populations of Citrus tristeza virus isolates, Phytopathology, 90, pp. 134-138, (2000)

[2]

Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J., Basic local alignment search tool, J Mol Biol, 215, pp. 403-410, (1990)

[3]

Ayllon M.A., Rubio L., Moya A., Guerri J., Moreno P., The haplotype distribution of two genes of citrus tristeza virus is altered after host change or aphid transmission, Virology, 255, pp. 32-39, (1999)

[4]

Bar-Joseph M., Lee R.F., Citrus Tristeza Virus. AAB Description of Plant Viruses No. 353, (1989)

[5]

Bove J.M., Virus and Virus-like Disease of Citrus in the Near-east Region, pp. 149-173, (1995)

[6]

Brandt J., Bylsma F.W., Gross R., Stine O.C., Ranen N., Ross C.A., Trinucleotide repeat length and clinical progression in Huntington's disease, Neurology, 46, pp. 527-531, (1996)

[7]

Burland T.G., DNASTAR's lasergene sequence analysis software, Methods Mol Biol, 132, pp. 71-91, (2000)

[8]

Cambra M., Gorris M.T., Roman M.P., Terrada E., Garnsey S.M., Camarasa E., Olmos A., Colomer M., Routine detection of citrus tristeza virus by direct immunoprinting-ELISA method using specific monoclonal and recombinant antibodies, Proc 14th Conf Inter Organ Citrus Virol, pp. 34-41, (2000)

[9]

Cevik B., Pappu S.S., Pappu H.R., Tight D., Benscher D., Futch S.H., Rucks P., Lee R.F., Niblett C.L., Molecular cloning and sequencing of coat protein genes of citrus tristeza virus isolated from Meyer lemon and Homely Tangor trees in Florida, Proc 13th Conf Intern Organ Citrus Virol, pp. 47-53, (1996)

[10]

D'Urso F., Ayllon M.A., Rubio L., Sambade A., Hermoso De Mendoza A., Guerri J., Moreno P., Contribution of uneven distribution of genomic RNA variants of citrus tristeza virus (CTV) within the plant to changes in the viral population following aphid transmission, Plant Pathol, 49, pp. 288-294, (2000)

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