Neuroprotective Effect of Cyclosporine Against Oxidative Stress-Induced Neurotoxicity in PC12 Cells

Oxidative stress has crucial role in neuronal cell death associated with neurodegenerative diseases including Alzheimer's disease. Excessive levels of reactive oxygen species (ROS) and induction of apoptosis are thought to lead to neuronal cell death. The aim of this study was to evaluate the effect of cyclosporine A (CsA) on hydrogen peroxide (H2O2)-elicited oxidative damage in PC12 cells. The cells were incubated with CsA and then treated with H2O2 for 24 h. The viability of cells was measured by MTT assay. Moreover, lipid peroxidation (LPO), total thiol molecules (TTM), and total antioxidant capacity (TAC) were evaluated. Our results demonstrated that CsA notably improved the reduced viability of PC12 cells induced by H2O2. Treating PC12 cells with H2O2 increased LPO level, whereas CsA significantly reduced LPO. CsA effectively increased the TTM and TAC level, as compared with H2O2 group. These findings suggested that CsA has beneficial effects against H2O2-mediated damage in PC12 cells. © The Author(s), under exclusive licence to The National Academy of Sciences, India 2023.