Immunoisolation of stem cells by simultaneous encapsulation and PEGylation

被引：10

作者：

Ramezanzadeh Andevari R. ^{[1
]}

Hashemi-Najafabadi S. ^{[1
]}

Bagheri F. ^{[2
]}

机构：

[1] Department of Biomedical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran

[2] Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran

来源：

Progress in Biomaterials | 2018年 / 7卷 / 1期

关键词：

Alginate; Embryonic stem cells (ESCs); Microencapsulation; PEGylation; Trimethyl chitosan (TMC);

D O I：

10.1007/s40204-018-0084-3

中图分类号：

学科分类号：

摘要：

Today, cell therapy is known as an important tool in the treatment of chronic diseases where cells lose their normal function. Immunoisolation systems using microencapsulation or PEGylation have been developed to evade the problem of rejection by the immune system. The aim of the present study was to investigate a combination of microencapsulation and PEGylation methods in coating mouse embryonic stem cells (mESCs) to determine its effect in reducing the host’s immune response. Therefore, methoxy polyethylene glycol (mPEG) binding on alginate–trimethyl chitosan (TMC) microcapsules was investigated using FTIR. Furthermore, survival of the microencapsulated mESCs was confirmed using AO/PI staining and MTT assays. In addition, the effect of mESCs co-cultured with foreign lymphocytes was evaluated. Overall, interleukin-2 (IL-2) secretions as a response of the immune system revealed that mESCs microencapsulation in alginate–TMC–PEG, reduced the immune system response. The results suggested that IL-2 secretion was reduced to 62% at seventh day. © 2018, The Author(s).

引用

页码：55 / 60

页数：5

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