Indole-3-acetic acid reverses the harpin-induced hypersensitive response and alters the expression of hypersensitive-response-related genes in tobacco

被引：0
作者：
Jie Song
Xiao-chong Gong
Wei-guo Miao
Fu-cong Zheng
Cong-feng Song
Ming-hua Wang
机构：
[1] Hainan University,Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource/College of Environment and Plant Protection
[2] Nanjing Agricultural University,Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education/College of Plant Protection
来源：
Biotechnology Letters | 2014年 / 36卷
关键词：
Disease-defense gene; Harpin; Hypersensitive response; Indole-3-acetic acid; Regulation of the hypersensitive response mechanism; Tobacco; Triiodobenzoic acid;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Harpin proteins stimulate hypersensitive response (HR) in plants. However, the mechanism by which HR is regulated is not clear. The role of the auxin, indole-3-acetic acid (IAA), in the control of harpin-stimulated HR was investigated. IAA was used to inhibit HR that was stimulated by purified fusion harpinXoo protein in tobacco. Semi-quantitative PCR and qRT-PCR were employed to detect the expression of HR related genes. IAA at 100 μM reversed harpin-induced HR which was inhibited by 500 μM 2,3,5-triiodobenzoic acid (TIBA). Semi-quantitative PCR and qRT-PCR showed the combined application of 100 μM IAA and harpin protein from Xanthomonas oryzae enhanced the expression of HR marker gene, hsr203J, but weakened the expression of the disease-defense gene, chia5. TIBA also decreased the expression of hsr203J but increased the expression of chia5. Thus, the auxin can reverse harpinXoo-induced HR.
引用
收藏
页码：1043 / 1048
页数：5
相关论文
共 40 条
  • [1] Andi S(2001)Effect of methyl jasmonate on harpin-induced hypersensitive cell death, generation of hydrogen peroxide and expression of PAL mRNA in tobacco suspension cultured BY-2 cells Plant Cell Physiol 42 446-449
  • [2] Taguchi F(2008)Reversal of an immunity associated plant cell death program by the growth regulator auxin BMC Res Notes 1 126-563
  • [3] Toyoda K(1994)Programmed cell death in plants: a pathogen-triggered response activated coordinately with multiple defense functions Cell 77 551-616
  • [4] Gopalan S(1996)The interaction of harpinPss, with plant cell walls MPMI 9 608-1093
  • [5] Greenberg JT(2001)A harpin binding site in tobacco plasma membranes mediates ctivation of the pathogenesis-related gene HIN1 independent of extracellular calcium but dependent on mitogen-activated protein kinase activity Plant Cell Online 13 1079-66
  • [6] Guo A(2005)Harpin of Mol Plant-Microbe Interact 18 60-1420
  • [7] Klessig DF(2010) pv. phaseolicola harbors a protein binding site BMC Plant Biol 10 67-343
  • [8] Hoyos ME(2005)Genetic transformation of cotton with a harpin-encoding gene hpaXoo confers an enhanced defense response against different pathogens through a priming mechanism Mol Microbiol 58 1406-5749
  • [9] Stanley CM(2011)Ca Ann Rev Phytopathol 49 317-189
  • [10] He SY(1990)-dependent lipid binding and membrane integration of PopA, a harpin-like elicitor of the hypersensitive response in tobacco J Bacteriol 172 5742-239
1234