Overexpression of Collapsin Response Mediator Protein 1 Inhibits Human Trophoblast Cells Proliferation, Migration, and Invasion

被引:3
作者
Huang, Ling [1 ,2 ,3 ]
Li, Yuanyuan [1 ,2 ,3 ]
Wang, Chunhui [4 ]
Li, Na [1 ,2 ,3 ,5 ]
Hou, Yue [1 ,2 ,3 ]
Chang, Ruijing [6 ]
Sun, Man [1 ,2 ,3 ]
Wang, Ruochen [7 ]
Zhu, Lei [8 ]
Qiao, Chong [1 ,2 ,3 ]
机构
[1] China Med Univ, Shengjing Hosp, Dept Obstet & Gynecol, Shenyang, Liaoning, Peoples R China
[2] China Med Univ, Key Lab Maternal Fetal Med, Shenyang, Liaoning, Peoples R China
[3] China Med Univ, Key Lab Obstet & Gynecol Higher Educ, Shenyang, Liaoning, Peoples R China
[4] Gen Hosp Shenyang Mil Reg, Dept Hepatobiliary Surg, Shenyang, Liaoning, Peoples R China
[5] Shenyang Forth Peoples Hosp, Dept Obstet & Gynecol, Shenyang, Liaoning, Peoples R China
[6] Hebei Med Univ, Hosp 2, Dept Obstet & Gynecol, Shijiazhuang, Hebei, Peoples R China
[7] Univ Wisconsin, Dept Biomed Engn, Madison, WI USA
[8] Shenyang Acad Environm Sci, Shenyang, Liaoning, Peoples R China
基金
国家重点研发计划; 中国国家自然科学基金;
关键词
collapsin response mediator protein 1 (CRMP-1); preeclampsia; trophoblast cells; MATRIX METALLOPROTEINASES; PREECLAMPSIA; EXPRESSION; MORPHOGENESIS;
D O I
10.1177/1933719118799214
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Collapsin response mediator protein 1 (CRMP-1) is widely expressed in the nervous system and has tumor suppressive effects. Our previous studies have demonstrated that CRMP-1 was expressed in the trophoblasts of the whole stage of pregnancy with significantly increasing expression in the placenta of early-onset preeclampsia. Preeclampsia, especially early onset, is strongly associated with the dysfunction of trophoblast including proliferation, apoptosis, migration, and invasion. In this study, we found an inhibitory effect of CRMP-1 on proliferation, migration, invasion, and an enhanced effect on apoptosis in human trophoblast cell lines HTR-8/SVneo and JEG-3 by MTT assay, colony formation assay, cell viability assay, caspase 3/7 activity assay, scratch wound assay, and Matrigel Transwell assay. Overexpression of CRMP-1 in trophoblast cells led to downregulate expression of matrix metalloproteinase 2 and 9. The expression of CRMP-1 was detected by real-time quantitative polymerase chain reaction and Western blot analysis. Thus, we suggested that CRMP-1 might have implications for the pathogenesis of preeclampsia by regulating the biological behavior of trophoblast cells.
引用
收藏
页码:954 / 960
页数:7
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