Overexpression of Collapsin Response Mediator Protein 1 Inhibits Human Trophoblast Cells Proliferation, Migration, and Invasion

被引:3
作者
Huang, Ling [1 ,2 ,3 ]
Li, Yuanyuan [1 ,2 ,3 ]
Wang, Chunhui [4 ]
Li, Na [1 ,2 ,3 ,5 ]
Hou, Yue [1 ,2 ,3 ]
Chang, Ruijing [6 ]
Sun, Man [1 ,2 ,3 ]
Wang, Ruochen [7 ]
Zhu, Lei [8 ]
Qiao, Chong [1 ,2 ,3 ]
机构
[1] China Med Univ, Shengjing Hosp, Dept Obstet & Gynecol, Shenyang, Liaoning, Peoples R China
[2] China Med Univ, Key Lab Maternal Fetal Med, Shenyang, Liaoning, Peoples R China
[3] China Med Univ, Key Lab Obstet & Gynecol Higher Educ, Shenyang, Liaoning, Peoples R China
[4] Gen Hosp Shenyang Mil Reg, Dept Hepatobiliary Surg, Shenyang, Liaoning, Peoples R China
[5] Shenyang Forth Peoples Hosp, Dept Obstet & Gynecol, Shenyang, Liaoning, Peoples R China
[6] Hebei Med Univ, Hosp 2, Dept Obstet & Gynecol, Shijiazhuang, Hebei, Peoples R China
[7] Univ Wisconsin, Dept Biomed Engn, Madison, WI USA
[8] Shenyang Acad Environm Sci, Shenyang, Liaoning, Peoples R China
基金
国家重点研发计划; 中国国家自然科学基金;
关键词
collapsin response mediator protein 1 (CRMP-1); preeclampsia; trophoblast cells; MATRIX METALLOPROTEINASES; PREECLAMPSIA; EXPRESSION; MORPHOGENESIS;
D O I
10.1177/1933719118799214
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Collapsin response mediator protein 1 (CRMP-1) is widely expressed in the nervous system and has tumor suppressive effects. Our previous studies have demonstrated that CRMP-1 was expressed in the trophoblasts of the whole stage of pregnancy with significantly increasing expression in the placenta of early-onset preeclampsia. Preeclampsia, especially early onset, is strongly associated with the dysfunction of trophoblast including proliferation, apoptosis, migration, and invasion. In this study, we found an inhibitory effect of CRMP-1 on proliferation, migration, invasion, and an enhanced effect on apoptosis in human trophoblast cell lines HTR-8/SVneo and JEG-3 by MTT assay, colony formation assay, cell viability assay, caspase 3/7 activity assay, scratch wound assay, and Matrigel Transwell assay. Overexpression of CRMP-1 in trophoblast cells led to downregulate expression of matrix metalloproteinase 2 and 9. The expression of CRMP-1 was detected by real-time quantitative polymerase chain reaction and Western blot analysis. Thus, we suggested that CRMP-1 might have implications for the pathogenesis of preeclampsia by regulating the biological behavior of trophoblast cells.
引用
收藏
页码:954 / 960
页数:7
相关论文
共 28 条
[11]   CRMP1 Inhibits Proliferation of Medulloblastoma and Is Regulated by HMGA1 [J].
Li, Kay Ka-Wai ;
Qi, Yan ;
Xia, Tian ;
Yao, Yu ;
Zhou, Liangfu ;
Lau, Kin-Mang ;
Ng, Ho-Keung .
PLOS ONE, 2015, 10 (05)
[12]   Disruption of sphingolipid metabolism augments ceramide-induced autophagy in preeclampsia [J].
Melland-Smith, Megan ;
Ermini, Leonardo ;
Chauvin, Sarah ;
Craig-Barnes, Hayley ;
Tagliaferro, Andrea ;
Todros, Tullia ;
Post, Martin ;
Caniggia, Isabella .
AUTOPHAGY, 2015, 11 (04) :653-669
[13]   MicroRNA-30a-3p is overexpressed in the placentas of patients with preeclampsia and affects trophoblast invasion and apoptosis by its effects on IGF-1 [J].
Niu, Zi-Ru ;
Han, Tao ;
Sun, Xiao-Luan ;
Luan, Li-Xia ;
Gou, Wen-Li ;
Zhu, Xiao-Ming .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2018, 218 (02) :249.e1-249.e12
[14]   Placental and trophoblastic in vitro models to study preventive and therapeutic agents for preeclampsia [J].
Orendi, K. ;
Kivity, V. ;
Sammar, M. ;
Grimpel, Y. ;
Gonen, R. ;
Meiri, H. ;
Lubzens, E. ;
Huppertz, B. .
PLACENTA, 2011, 32 :S49-S54
[15]   Genetics of preeclampsia: paradigm shifts [J].
Oudejans, Cees B. M. ;
van Dijk, Marie ;
Oosterkamp, Marjet ;
Lachmeijer, Augusta ;
Blankenstein, Marinus A. .
HUMAN GENETICS, 2007, 120 (05) :607-612
[16]  
Qiao C, 2005, ZHONGHUA FU CHAN KE, V40, P13
[17]   Expression of Collapsin Response Mediator Protein 1 in Placenta of Normal Gestation and Link to Early-Onset Preeclampsia [J].
Qiao, Chong ;
Wang, Chunhui ;
Jin, Feng ;
Zheng, Dongying ;
Liu, Caixia .
REPRODUCTIVE SCIENCES, 2015, 22 (04) :495-501
[18]  
Qiao Chong, 2011, Journal of China Medical University, V40, P293
[19]   CRMPs: critical molecules for neurite morphogenesis and neuropsychiatric diseases [J].
Quach, T. T. ;
Honnorat, J. ;
Kolattukudy, P. E. ;
Khanna, R. ;
Duchemin, A. M. .
MOLECULAR PSYCHIATRY, 2015, 20 (09) :1037-1045
[20]   Imbalance between matrix metalloproteinases and their tissue inhibitors in preeclampsia and gestational trophoblastic diseases [J].
Rahat, Beenish ;
Sharma, Renuka ;
Bagga, Rashmi ;
Hamid, Abid ;
Kaur, Jyotdeep .
REPRODUCTION, 2016, 152 (01) :11-22