CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

被引：0
|
作者：
Qi Liu
Xiaona Shi
Lili Song
Haifeng Liu
Xiangshan Zhou
Qiyao Wang
Yuanxing Zhang
Menghao Cai
机构：
[1] East China University of Science and Technology,State Key Laboratory of Bioreactor Engineering
[2] Chinare Resources Angde Biotech Pharmaceutical Co.,undefined
[3] Ltd.,undefined
[4] Shanghai Collaborative Innovation Center for Biomanufacturing,undefined
来源：
Microbial Cell Factories | / 18卷
关键词：
CRISPR–Cas9; Homology directed repair; Multiloci integration; Multistep enzymatic pathway;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
引用
收藏
相关论文
共 50 条
  • [21] Development of CRISPR/Cas9-Mediated Gene-Drive Construct Targeting the Phenotypic Gene in Plutella xylostella
    Asad, Muhammad
    Liu, Dan
    Li, Jianwen
    Chen, Jing
    Yang, Guang
    FRONTIERS IN PHYSIOLOGY, 2022, 13
  • [22] An efficient platform for generating somatic point mutations with germline transmission in the zebrafish by CRISPR/Cas9-mediated gene editing
    Zhang, Yibo
    Zhang, Zhiwei
    Ge, Wei
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2018, 293 (17) : 6611 - 6622
  • [23] An Efficient Marker Gene Excision Strategy Based on CRISPR/Cas9-Mediated Homology-Directed Repair in Rice
    Tan, Jiantao
    Wang, Yaxi
    Chen, Shuifu
    Lin, Zhansheng
    Zhao, Yanchang
    Xue, Yang
    Luo, Yuyu
    Liu, Yao-Guang
    Zhu, Qinlong
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (03)
  • [24] MDM2 antagonists promote CRISPR/Cas9-mediated precise genome editing in sheep primary cells
    Li, Yan
    Lian, Di
    Wang, Jiahao
    Zhao, Yue
    Li, Yao
    Liu, Guoshi
    Wu, Sen
    Deng, Shoulong
    Du, Xuguang
    Lian, Zhengxing
    MOLECULAR THERAPY NUCLEIC ACIDS, 2023, 31 : 309 - 323
  • [25] Inhibition of DNA ligase IV enhances the CRISPR/Cas9-mediated knock-in efficiency in mouse brain neurons
    Cao, Xueshan
    Kouyama-Suzuki, Emi
    Pang, Bo
    Kurihara, Taiga
    Mori, Takuma
    Yanagawa, Toru
    Shirai, Yoshinori
    Tabuchi, Katsuhiko
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2020, 533 (03) : 449 - 457
  • [26] Efficient and Precise CRISPR/Cas9-Mediated MECP2 Modifications in Human-Induced Pluripotent Stem Cells
    Thi Thanh Huong Le
    Ngoc Tung Tran
    Thi Mai Lan Dao
    Dinh Dung Nguyen
    Huy Duong Do
    Thi Lien Ha
    Kuhn, Ralf
    Thanh Liem Nguyen
    Rajewsky, Klaus
    Van Trung Chu
    FRONTIERS IN GENETICS, 2019, 10
  • [27] DNA repair protein RAD51 enhances the CRISPR/Cas9-mediated knock-in efficiency in brain neurons
    Kurihara, Taiga
    Kouyama-Suzuki, Emi
    Satoga, Michiru
    Li, Xue
    Badawi, Moataz
    Thiha
    Baig, Deeba Noreen
    Yanagawa, Toru
    Uemura, Takeshi
    Mori, Takuma
    Tabuchi, Katsuhiko
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2020, 524 (03) : 621 - 628
  • [28] CRISPR/Cas9-mediated knock-in of alligator cathelicidin gene in a non-coding region of channel catfish genome
    Simora, Rhoda Mae C.
    Xing, De
    Bangs, X. R.
    Wang, Wenwen
    Ma, Xiaoli
    Su, Baofeng
    Khan, Mohd G. Q.
    Qin, Zhenkui
    Lu, Cuiyu
    Alston, Veronica
    Hettiarachchi, Darshika
    Johnson, Andrew
    Li, Shangjia
    Coogan, Michael
    Gurbatow, Jeremy
    Terhune, Jeffery S.
    Wang, Xu
    Dunham, Rex A.
    SCIENTIFIC REPORTS, 2020, 10 (01)
  • [29] Validation Study to Determine the Accuracy of Widespread Promoterless EGFP Reporter at Assessing CRISPR/Cas9-Mediated Homology Directed Repair
    Xu, Wanqing
    Zuo, Qingxia
    Feng, Dongyan
    He, Changsheng
    Lin, Cailing
    Huang, Dongchao
    Wan, Yanbin
    Chen, Feng
    Mo, Guosheng
    Sun, Qi
    Du, Hongli
    Huang, Lizhen
    CURRENT ISSUES IN MOLECULAR BIOLOGY, 2022, 44 (04) : 1688 - 1700
  • [30] Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage
    Zhang, Jian-Ping
    Li, Xiao-Lan
    Li, Guo-Hua
    Chen, Wanqiu
    Arakaki, Cameron
    Botimer, Gary D.
    Baylink, David
    Zhang, Lu
    Wen, Wei
    Fu, Ya-Wen
    Xu, Jing
    Chun, Noah
    Yuan, Weiping
    Cheng, Tao
    Zhang, Xiao-Bing
    GENOME BIOLOGY, 2017, 18
12345