The Role of Stretch-Activated Channels in Mediating mTORC1 Signaling in Isolated Rat Soleus Muscle in Response to Mechanical Stimulation after Functional Unloading

被引：0

作者：

Mirzoev T.M. ^{[1
]}

Tyganov S.A. ^{[1
]}

Shenkman B.S. ^{[1
]}

机构：

[1] Institute of Medical Biological Problems, Russian Academy of Sciences, Moscow

来源：

Neuroscience and Behavioral Physiology | 2019年 / 49卷 / 6期

关键词：

4E-BP1; antiorthostatic suspension; eccentric contractions; mTORC1; p70S6k; SAC; soleus muscle;

D O I：

10.1007/s11055-019-00798-w

中图分类号：

学科分类号：

摘要：

The aim of the present work was to identify the role of stretch-activated ion channels (SAC) in the transmission of mechanical signals to the key mTORC1/p70S6k anabolic pathways in the soleus muscle in rats after seven days of functional unloading of the hindlimbs. Functional unloading of the hindlimb muscles was by antiorthostatic suspension (hindlimb suspension, HS). The rats were divided into four groups: 1) group C (animal-house control; 2) C + Gd (controls whose muscles were incubated with the SAC inhibitor GdCl3; 3) HS (functional unloading for seven days); and 4) HS + Gd (a group with seven days of antiorthostatic suspension followed by incubation of muscles with GdCl3). After completion of antiorthostatic suspension, isolated soleus muscles were subjected to a series of eccentric contractions (EC). Muscles from intact animals responded to EC with increases in the phosphorylation of key markers of mTORC1 signaling: p70S6k and rpS6. The extent of mechanically induced increases in the levels of phosphorylation of these protein markers were significantly reduced to essentially identical extents in the “pure” suspension (HS) group and the HS group given the SAC blocker (HS + Gd) as compared with the increase in muscles from intact control group. These results lead to the conclusion that decreases in the mechanically dependent responses of the mTORC1 signal pathway in the soleus muscle in rats after seven days of functional unloading may be linked with inactivation of stretch-activated ion channels. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.

引用

页码：759 / 764

页数：5

共 24 条

[1]

Tyganov S.A., Petrova I.O., Mirzoev T.M., Shenkman B.S., Changes in protein synthesis in isolated rat soleus muscle in response to eccentric loading on the background of antiorthostatic suspension, Aviakosmich. Ekol. Med., (2017)

[2]

Ato S., Makanae Y., Kido K., Fujita S., Contraction mode itself does not determine the level of mTORC1 activity in rat skeletal muscle, Physiol. Rep, 4, 19, (2016)

[3]

Bajotto G., Sato Y., Kitaura Y., Shimomura Y., Effect of branched-chain amino acid supplementation during unloading on regulatory components of protein synthesis in atrophied soleus muscles, Eur. J. Appl. Physiol., 111, 8, pp. 1815-1828, (2011)

[4]

Burry M., Hawkins D., Spangenburg E.E., Lengthening contractions differentially affect p70s6k phosphorylation compared to isometric contractions in rat skeletal muscle, Eur. J. Appl. Physiol., 100, pp. 409-415, (2007)

[5]

Cannavino J., Brocca L., Sandri M., Et al., PGC1-α over-expression prevents metabolic alterations and soleus muscle atrophy in hindlimb unloaded mice, J. Physiol., 592, 20, pp. 4575-4589, (2014)

[6]

Dupont E., Cieniewski-Bernard C., Bastide B., Stevens L., Electrostimulation during hindlimb unloading modulates PI3KAKT downstream targets without preventing soleus atrophy and restores slow phenotype through ERK, Am. J. Physiol. Regul. Integr. Comp. Physiol., 300, pp. 408-417, (2011)

[7]

Franco A., Winegar B.D., Lansman J.B., Open channel block by gadolinium ion of the stretch-inactivated ion channel in mdx myotubes, Biophys. J., 59, pp. 1164-1170, (1991)

[8]

Glass D.J., Signalling pathways that mediate skeletal muscle hypertrophy and atrophy, Nat. Cell Biol., 5, pp. 87-90, (2003)

[9]

Goldberg A.L., Etlinger J.D., Goldspink D.F., Jablecki C., Mechanism of work-induced hypertrophy of skeletal muscle, Med. Sci. Sports, 7, 3, pp. 185-198, (1975)

[10]

Gollnick P.D., Timson B.F., Moore R.L., Riedy M., Muscular enlargement and number of fibers in skeletal muscles of rats, J. Appl. Physiol. Respir. Environ. Exerc. Physiol., 50, pp. 936-943, (1981)

← 1 2 3 →