Effect of Lipoprotein-X on lipid metabolism in rat kidney

被引：0
作者：
O Karmin
May Ly
Ding Z. Fang
Jiri Frohlich
Patrick C. Choy
机构：
[1] St. Paul's Hospital,Department of Pathology and Laboratory Medicine, University of British Columbia, Atherosclerosis Specialty Laboratory
[2] University of Manitoba,Department of Biochemistry and Molecular Biology
来源：
Molecular and Cellular Biochemistry | 1997年 / 173卷
关键词：
Lipoprotein-X; LCAT deficiency; kidneyperfusion; cholesterol; choelsteryl esters; phosphatidycholine;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Lipoprotein-X (Lp-X) is found in the plasma of patients with familiallecithin: cholesterol acyltransferase (LCAT) deficiency syndromes. Themajority of the patients with this disorder develop progressiveglomerulosclerosis. In this study, the effect of Lp-X on lipid metabolism inperfused rat kidney was investigated. Lp-X was isolated from plasma ofpatients with familial LCAT deficiency by sequential ultracentrifugation andgel filtration column chromatography. Rat kidneys were perfused for 1-2 hwith Krebs-Henseleit buffer containing 20 µM [1-14C]acetate or 20µM [Me-3H]choline. In the presence of Lp-X, no significant differencein the incorporation of radioactivity into triglycerides, cholesterol,phosphocholine, CDP-choline and sphingomyelin was observed. However,incorporation of radioactivity into cholesteryl esters andphosphatidylcholine was significantly elevated in Lp-X perfused kidneys. Thecontents of cholesterol, cholesteryl esters and phosphatidylcholine werealso significantly increased in Lp-X perfused kidneys. The increase in lipidcontent in the Lp-X perfused kidney is attributed to the direct depositionof Lp-X lipids into the organ. The increase in the labelling of cholesterylesters was attributed to the increase of available substrate (cholesterol)for the acyl-CoA:cholesterol acyltransferase (ACAT) reaction. The increasein phosphatidylcholine labelling was caused by a reduced turnover of thenewly synthesized labelled phosphatidylcholine during Lp-X perfusion.
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页码：17 / 24
页数：7
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