Functional Role of C-terminal Domains in the MSL2 Protein of Drosophila melanogaster

被引：1

作者：

Tikhonova, Evgeniya A. ^{[1
]}

Georgiev, Pavel G. ^{[1
]}

Maksimenko, Oksana G. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Gene Biol, Moscow 119334, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2024年 / 89卷 / 04期

关键词：

dosage compensation; long non-coding RNAs; MSL1; roX; MSL complex; ubiquitination; DOSAGE COMPENSATION COMPLEX; MALE-SPECIFIC LETHAL-2; SEX-SPECIFIC REGULATION; X-CHROMOSOME; RING FINGER; STRUCTURAL BASIS; CXC DOMAIN; GENE; BINDING; MOF;

D O I：

10.1134/S0006297924040060

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dosage compensation complex (DCC), which consists of five proteins and two non-coding RNAs roX, specifically binds to the X chromosome in males, providing a higher level of gene expression necessary to compensate for the monosomy of the sex chromosome in male Drosophila compared to the two X chromosomes in females. The MSL2 protein contains the N-terminal RING domain, which acts as an E3 ligase in ubiquitination of proteins and is the only subunit of the complex expressed only in males. Functional role of the two C-terminal domains of the MSL2 protein, enriched with proline (P-domain) and basic amino acids (B-domain), was investigated. As a result, it was shown that the B-domain destabilizes the MSL2 protein, which is associated with the presence of two lysines ubiquitination of which is under control of the RING domain of MSL2. The unstructured proline-rich domain stimulates transcription of the roX2 gene, which is necessary for effective formation of the dosage compensation complex.

引用

页码：663 / 673

页数：11

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