Thermotolerant Acanthamoeba spp. isolated from recreational water in Gorgan City, north of Iran

被引：1
作者：
Maghsoodloorad S. [1 ]
Maghsoodloorad E. [2 ]
Tavakoli Kareshk A. [3 ]
Motazedian M.H. [4 ]
Yusuf M.A. [5 ]
Solgi R. [3 ]
机构：
[1] Department of Parasitology and Mycology, School of Medicine, Golestan University of Medical Science, Gorgān
[2] Sayyad Shirazi Hospital, Golestan University of Medical Science, Gorgān
[3] Infectious Disease Research Center, Birjand University of Medical Sciences, Birjand
[4] Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz
[5] Department of Medical Entomology and Vector Control, School of Public Health, International Campus, Tehran University of Medical Science, Tehran
来源：
Journal of Parasitic Diseases | 2019年 / 43卷 / 2期
关键词：
Acanthamoeba; Genotyping techniques; Iran; Molecular; Morphological;
D O I：
10.1007/s12639-018-01081-4
中图分类号：
学科分类号：
摘要：
Acanthamoeba as free-living parasites are scattered ubiquitously, throughout the world. This study was aimed to evaluate the presence of Acanthamoeba spp. genotypes in the recreational water sources in Gorgan County, the capital of Golestan Province using both morphological and molecular approaches. Thirty water samples were collected from different recreational waters in Gorgan, the capital of Golestan Province, northern Iran during 2015–2016. Samples were filtered and followed by culture in non-nutrient agar. Acanthamoeba were identified both by morphological and molecular analysis. The pathogenical potential of positive cloned samples were also determined using tolerance test. Twenty-six percent of recreational water were identified as Acanthamoeba spp. based on the morphological analysis and from these positive samples, five samples were successfully sequenced after molecular studies. Phylogenetic analysis showed the clustering of four samples in T4 genotype group and only one sample as T15 genotype. Thermotolerance test revealed that all cloned samples were highly positive. Since the attractiveness of recreational places for people is increasing, the potential risk of this water should be monitored routinely in each region. More studies are needed to better evaluate the risk of this ubiquitous parasite for the human. © 2019, Indian Society for Parasitology.
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页码：240 / 245
页数：5
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