Identification of QTLs conferring resistance to downy mildew in legacy cultivars of lettuce

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作者：
Ivan Simko
Amy J. Atallah
Oswaldo E. Ochoa
Rudie Antonise
Carlos H. Galeano
Maria Jose Truco
Richard W. Michelmore
机构：
[1] Agricultural Research Service,U.S. Department of Agriculture
[2] U.S. Agricultural Research Station,The Genome Center and Department of Plant Sciences
[3] University of California,undefined
[4] KeyGene N.V.,undefined
来源：
Scientific Reports | / 3卷
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摘要：
Many cultivars of lettuce (Lactuca sativa L.), the most popular leafy vegetable, are susceptible to downy mildew disease caused by Bremia lactucae. Cultivars Iceberg and Grand Rapids that were released in the 18th and 19th centuries, respectively, have high levels of quantitative resistance to downy mildew. We developed a population of recombinant inbred lines (RILs) originating from a cross between these two legacy cultivars, constructed a linkage map and identified two QTLs for resistance on linkage groups 2 (qDM2.1) and 5 (qDM5.1) that determined resistance under field conditions in California and the Netherlands. The same QTLs determined delayed sporulation at the seedling stage in laboratory experiments. Alleles conferring elevated resistance at both QTLs originate from cultivar Iceberg. An additional QTL on linkage group 9 (qDM9.1) was detected through simultaneous analysis of all experiments with mixed-model approach. Alleles for elevated resistance at this locus originate from cultivar Grand Rapids.
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