Exploring the antifungal, antibiofilm and antienzymatic potential of Rottlerin in an in vitro and in vivo approach

被引：5

作者：

Sousa Silva, Nagela Bernadelli ^{[1
]}

Menezes, Ralciane Paula ^{[2
]}

Goncalves, Daniela Silva ^{[1
]}

Santiago, Mariana Brentini ^{[1
]}

Conejo, Noemi Chagas ^{[1
]}

Souza, Sara Lemes ^{[1
]}

Oliveira Santos, Anna Livia ^{[1
]}

da Silva, Robinson Sabino ^{[3
]}

Ramos, Salvador Boccaletti ^{[4
]}

Vieira Ferro, Eloisa Amalia ^{[5
]}

Gomes Martins, Carlos Henrique ^{[1
]}

机构：

[1] Fed Univ Uberlandia UFU, Inst Biomed Sci ICBIM, Lab Antimicrobial Testing, Ave Para 1720, BR-17203840 Uberlandia, MG, Brazil

[2] Fed Univ Uberlandia UFU, Tech Sch Hlth ESTES, Uberlandia, MG, Brazil

[3] Fed Univ Uberlandia UFU, Inst Biomed Sci ICBIM, Innovat Ctr Salivary Diagnost & Nanotheranost, Uberlandia, MG, Brazil

[4] SAo Paulo State Univ UNESP, Fac Agr & Vet Sci Jaboticabal FCAV, Dept Engn & Exact Sci, Jaboticabal, Brazil

[5] Univ Fed Uberlandia, Inst Biomed Sci ICBIM, Lab Immunophysiol Reprod, Uberlandia, MG, Brazil

来源：

SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期

关键词：

Rottlerin; Antifungal activity; Antibiofilm; Hydrolytic enzymes; Caenorhabditis elegans; CANDIDA; AUTOPHAGY; VIRULENCE; STRAINS; ELEGANS; GROWTH;

D O I：

10.1038/s41598-024-61179-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Candida species have been responsible for a high number of invasive infections worldwide. In this sense, Rottlerin has demonstrated a wide range of pharmacological activities. Therefore, this study aimed to evaluate the antifungal, antibiofilm and antivirulence activity of Rottlerin in vitro against Candida spp. and its toxicity and antifungal activity in vivo. Rottlerin showed antifungal activity against all yeasts evaluated, presenting Minimum Inhibitory and Fungicidal Concentration (MIC and MFC) values of 7.81 to>1000 mu g/mL. Futhermore, it was able to significantly inhibit biofilm production, presenting Biofilm Inhibitory Concentration (MICB50) values that ranged from 15.62 to 250 mu g/mL and inhibition of the cell viability of the biofilm by 50% (IC50) from 2.24 to 12.76 mu g/mL. There was a considerable reduction in all hydrolytic enzymes evaluated, with emphasis on hemolysin where Rottlerin showed a reduction of up to 20%. In the scanning electron microscopy (SEM) analysis, Rottlerin was able to completely inhibit filamentation by C. albicans. Regarding in vivo tests, Rottlerin did not demonstrate toxicity at the therapeutic concentrations demonstrated here and was able to increase the survival of C. elegans larvae infected. The results herein presented are innovative and pioneering in terms of Rottlerin's multipotentiality against these fungal infections.

引用

页数：14

共 59 条

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