Systematic optimisation of high-performance liquid chromatographic separation by varying the temperature, gradient, and stationary phase

被引：0

作者：

Matthias Otto

Anke Schirmer

Ute Claußnitzer

Michael Pfeffer

机构：

[1] Institute of Analytical Chemistry,

[2] Freiberg University of Mining and Technology,undefined

[3] 09596 Freiberg,undefined

[4] Germany,undefined

[5] Schering AG,undefined

[6] In-process Control,undefined

[7] 13342 Berlin,undefined

[8] Müllerstr. 178,undefined

[9] Germany,undefined

来源：

Analytical and Bioanalytical Chemistry | 2002年 / 372卷

关键词：

HPLC optimisation Column-switching Categorical variables;

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摘要：

Optimisation of the separation of a synthetic drug mixture by HPLC is performed by changing both continuous variables, i.e. mobile phase composition and temperature, and categorical variables, here the stationary phase. The retention of solutes is described on the basis of a general linear model in which the different columns are modelled by indicator variables. From the solute-specific retention models the global separation optimum is evaluated on the basis of multidimensional window diagrams using relative retentions of all peak pairs as the figure-of-merit.

引用

页码：341 / 346

页数：5