Protein renaturation with simultaneous purification by protein folding liquid chromatography: recent developments

被引：0
|
作者：
Lili Wang
Xindu Geng
机构：
[1] Northwest University,Key Laboratory of Synthetic and Natural Functional Molecular Chemistry of Ministry of Education, Shaanxi Key Laboratory of Modern Separation Science, Institute of Modern Separation Science
来源：
Amino Acids | 2014年 / 46卷
关键词：
Protein folding liquid chromatography (PFLC); Proteins; Inclusion bodies; Refolding with simultaneous purification; Mixed-mode liquid chromatography;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Protein folding liquid chromatography (PFLC) is a powerful tool for protein refolding with simultaneous purification. We review its recent progress in liquid chromatography and molecular biology, primarily involving the validation of PFLC refolding of proteins containing multiple disulphide bonds, the application of mixed-mode chromatography, PFLC in molecular biology. Representative examples are described.
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页码：153 / 165
页数：12
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