Methods to find out the expression of activated genes

被引：3
作者：
Cekan S.Z. [1 ]
机构：
[1] Karolinska Institute, Department of Woman and Child Health, Karolinska University Hospital, 17176 Stockholm
来源：
Reproductive Biology and Endocrinology | / 2卷 / 1期
关键词：
Differential Display; Test Preparation; Test cDNA; Representational Difference Analysis; Transcription Initiation Complex;
D O I：
10.1186/1477-7827-2-68
中图分类号：
学科分类号：
摘要：
This review deals with the methods of identifying genes that have been activated by inner or outer impulses. The activation and subsequent expression of a gene can be detected by its transcription into a corresponding messenger ribonucleic acid (mRNA). Principles of the methods for identification of individual activated genes, as well as groups of activated genes are described, the former methods being mostly based on subtractive hybridization and serial analysis of gene expression (SAGE), the latter on microarrays. Examples of gene activation by the hormone 17betaestradiol (E2) are given. © 2004 Cekan; licensee BioMed Central Ltd.
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页数：11
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