Fabrication and optimization of Nanodiamonds-composited poly(ε-caprolactone) fibrous matrices for potential regeneration of hard tissues

被引：17
作者：
Ahn G.Y. [1 ]
Ryu T.-K. [1 ]
Choi Y.R. [1 ]
Park J.R. [1 ]
Lee M.J. [1 ]
Choi S.-W. [1 ]
机构：
[1] The Catholic University of Korea, Department of Biotechnology, 43 Jibong-ro, Wonmi-gu, Bucheon-si, Gyeonggi-do
来源：
Biomaterials Research | / 22卷 / 1期
基金：
新加坡国家研究基金会;
关键词：
Biodegradable polymer; Composite; Electrospinning; Guided tissue engineering; Nanodiamond;
D O I：
10.1186/s40824-018-0126-x
中图分类号：
学科分类号：
摘要：
Background: Electrospun fibrous matrices are of great importance for tissue engineering and drug delivery device. However, relatively low mechanical strength of the fibrous matrix is one of the major disadvantages. NDs with a positive charge were selected to enhance the mechanical property of a composited fibrous matrix by inducing the intermolecular interaction between NDs and polymer chain. We prepared ND-composited poly (ε-caprolactone) (PCL) fibrous matrices by electrospinning and evaluated their performance in terms of mechanical strength and cell behaviors. Methods: A predetermined amounts of NDs (0.5, 1, 2 and 3 wt%) were added into PCL solution in a mixture of chloroform and 2,2,2-trifluoroethanol (8:2). ND-composited PCL (ND/PCL) fibrous matrices were prepared by electrospinning method. The tensile properties of the ND/PCL fibrous matrices were analyzed by using a universal testing machine. Mouse calvaria-derived preosteoblast (MC3T3-E1) was used for cell proliferation, alkaline phosphatase (ALP) assay, and Alizarin Red S staining. Results: The diameters of the fibrous matrices were adjusted to approximately 1.8 μm by changing process variables. The intermolecular interaction between NDs and PCL polymers resulted in the increased tensile strength and the favorable interfacial adhesion in the ND/PCL fibrous matrices. The ND/PCL fibrous matrix with 1 wt% of ND had the highest tensile strength among the samples and also improved proliferation and differentiation of MC3T3-E1 cells. Conclusions: Compared to the other samples, the ND/PCL fibrous matrix with 1 wt% of ND concentration exhibited superior performances for MC3T3 cells. The ND/PCL fibrous matrix can be potentially used for bone and dental tissue engineering. © 2018 The Author(s).
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