An enzyme-linked immunosorbent assay for diagnosis of Fasciola gigantica infection in cattle and buffaloes

被引：3
作者：
Krishna Murthy C.M. [1 ]
Souza P.E.D. [2 ]
机构：
[1] Department of Veterinary Parasitology, Veterinary College, Vinobanagar, Shimoga, 577204, Karnataka
[2] Centre of Advanced Faculty Training, Deartment of Veterinary Parasitology, Veterinary College, Hebbal, KVAFSU, Bangalore
来源：
Journal of Parasitic Diseases | 2015年 / 39卷 / 4期
关键词：
Excretory–secretory antigen; Fasciola gigantica; Sensitivity;
D O I：
10.1007/s12639-013-0414-2
中图分类号：
学科分类号：
摘要：
The enzyme-linked immunosorbent assay (ELISA) was evaluated for the diagnosis of Fasciola gigantica infection in cattle and buffaloes. The excretory–secretory (E–S Ag) antigen of F. gigantica adult flukes obtained after invitro incubation was used as an antigen. The test was conducted with 276 sera collected from cattle and buffaloes which included 22 sera each from naturally infected cattle and buffaloes (known positive serum) and with similar number of samples with healthy cattle and buffaloes (known negative serum). The positive results were observed in 18 and 19 of the sera from naturally infected cattle and buffaloes with sensitivity of 81.8 and 86.3 % respectively. Out of 188 serum samples which were found negative on faecal examination 32 (34 %) sera of cattle and 40 (42.5 %) sera of buffaloes were found positive by ELISA respectively. The sensitivity of the test was found to be 91.6 and 95.6 % in cattle and buffaloes respectively. © 2013, Indian Society for Parasitology.
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页码：783 / 785
页数：2
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