Synthesis of a Novel pH-Sensitive Methacrylate Amphiphilic Polymer and Its Primary Application in Aqueous Two-phase Systems

被引：0
作者：
Wei QIN
Xue-Jun CAO
机构：
[1] East China University of Science and Technology,State Key laboratory of Bioreactor Engineering, Department of Biochemical Engineering
来源：
Applied Biochemistry and Biotechnology | 2008年 / 150卷
关键词：
Aqueous two-phase systems; PH-sensitive; Amphiphilic polymer; Methacrylate; Polymer recycling;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this study, a novel pH-sensitive and reversible water-soluble polymer(PABC) forming aqueous two-phase systems(ATPS) was synthesized by using 2-(dimethylamino)ethyl methacrylate, t-butyl methacrylate, and methyl methacrylate as monomers and 2,2′-azo-bis-isobutyronitrile as initiator. The PABC could be recovered by adjusting isoelectric point (PI) to 8.4, and recovery at PI could reach 95%. ATPS was formed by 5% (w/w) PABC and 10% (w/w) PEG20000. The partition coefficient K of lysozyme was 6.8, and the partition coefficient K of bovine serum albumin could reach 12.5 in the ATPS.
引用
收藏
页码：171 / 183
页数：12
相关论文
共 73 条
  • [11] Persson J.(1992)Synthesis and application of 2-(dimethylamino) ethyl methacrylate Chemistry and Adhesion 1 29-736
  • [12] Nystrom L.(1993)Improvement on synthesis of 2-(dimethylamino) ethyl methacrylate Chemical Reagent 21 373-2871
  • [13] Ageland H.(2002)Preparation of 2-(dimethylamino) ethyl methacrylate Journal of Sichuan University 4 733-2148
  • [14] Tjermeld F.(1981)Kinetics of the anionic polymerization of tert-butyl methacrylate in tetrahydrofuran Makromolekulare Chemie 182 2863-414
  • [15] Johansson H. O.(1992)Syntheis, characterization and modification of poly(tert-butyl methacrylate-b-alkyl methacrylate-b-tert-butyl methacrylate) by group transfer polymerization[J] Journal of Polymer Science Part A 30 2143-19
  • [16] Persson J.(1993)Synthesis of monodisperse block copolymers containing methacrylic acid segments by group-transfer polymerization: Choice of protecting group and catalyst European Polymer Journal 79 407-937
  • [17] Tjerneld F.(1998)Syntheis, characterization and ion-polymerization of Journal of Beijing University of Chemical Technology 25 14-17
  • [18] Persson J.(1993)-butyl methacrylate Chinese Journal of Analysis Chemistry 21 1470-52
  • [19] Johansson H. O.(1994)The mesuration of PEG concentration in aqueous two-phase systems (ATPS) Macromolecules 27 930-194
  • [20] Tjerneld F.(1995)Diblock, ABC triblock, and random methacrylic polyampholytes: Synthesis by group transfer polymerization and solution behavior Biotechnology and Bioengineering 46 443-149
12345678