Synthesis of a Novel pH-Sensitive Methacrylate Amphiphilic Polymer and Its Primary Application in Aqueous Two-phase Systems

被引：0
作者：
Wei QIN
Xue-Jun CAO
机构：
[1] East China University of Science and Technology,State Key laboratory of Bioreactor Engineering, Department of Biochemical Engineering
来源：
Applied Biochemistry and Biotechnology | 2008年 / 150卷
关键词：
Aqueous two-phase systems; PH-sensitive; Amphiphilic polymer; Methacrylate; Polymer recycling;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this study, a novel pH-sensitive and reversible water-soluble polymer(PABC) forming aqueous two-phase systems(ATPS) was synthesized by using 2-(dimethylamino)ethyl methacrylate, t-butyl methacrylate, and methyl methacrylate as monomers and 2,2′-azo-bis-isobutyronitrile as initiator. The PABC could be recovered by adjusting isoelectric point (PI) to 8.4, and recovery at PI could reach 95%. ATPS was formed by 5% (w/w) PABC and 10% (w/w) PEG20000. The partition coefficient K of lysozyme was 6.8, and the partition coefficient K of bovine serum albumin could reach 12.5 in the ATPS.
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页码：171 / 183
页数：12
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