Expression of an alfalfa (Medicago sativa L.) ethylene response factor gene MsERF8 in tobacco plants enhances resistance to salinity

被引：0
作者：
Tingting Chen
Qingchuan Yang
Margaret Gruber
Junmei Kang
Yan Sun
Wang Ding
Tiejun Zhang
Xinquan Zhang
机构：
[1] China Agricultural University,Department of Grassland Science, College of Animal Science and Technology
[2] Chinese Academy of Agricultural Sciences,Institute of Animal Science
[3] Agriculture and Agri-Food Canada,Saskatoon Research Centre
[4] Sichuan Agricultural University,Department of Grassland Science, Animal Science and Technology College
来源：
Molecular Biology Reports | 2012年 / 39卷
关键词：
Alfalfa; Ethylene response factor gene; Expression analysis; Subcellular localization; Transgenic tobacco; Salt stress;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Ethylene response factors (ERF) play crucial roles in plant development and response to stresses. Here, a novel cDNA fragment (MsERF8) encoding an ERF protein with an AP2 domain was isolated and characterized from alfalfa. The MsERF8 cDNA has an open reading frame of 603 bp and encodes a nuclear protein of 201 amino acids. Q-RT-PCR analysis revealed that MsERF8 was strongly enriched in roots and leaves compared with stems, flower buds and flowers of mature alfalfa plants. Bioinformatic analysis of the MsERF8 promoter indicated a number of elements associated with stress-related responses, and MsERF8 transcripts in alfalfa seedlings were induced by NaCl, PEG6000, Al2(SO4)3 and five different hormones. Expression of MsERF8 in transgenic tobacco plants resulted in higher tolerance to salinity than with non-transgenic plants. This data shows that MsERF8 is a gene which prevents or alleviates salinity damage and has strong potential to impart salt tolerance to other crop plants.
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页码：6067 / 6075
页数：8
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