Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

被引：0

作者：

David Rodriguez-Temporal

Fernando Alcaide

Ivana Mareković

James Anthony O’Connor

Rebecca Gorton

Jakko van Ingen

An Van den Bossche

Genevieve Héry-Arnaud

Clémence Beauruelle

Dorothea Orth-Höller

Juan-José Palacios-Gutiérrez

Griselda Tudó

Germán Bou

Pieter-Jan Ceyssens

Montserrat Garrigó

Julià González-Martin

Gilbert Greub

Jaroslav Hrabak

André Ingebretsen

Maria Concepción Mediavilla-Gradolph

Marina Oviaño

Begoña Palop

Arthur B. Pranada

Lidia Quiroga

Maria Jesús Ruiz-Serrano

Belén Rodríguez-Sánchez

机构：

[1] Hospital General Universitario Gregorio Marañón,Department of Clinical Microbiology and Infectious Diseases

[2] Hospital Universitari de Bellvitge-IDIBELL,Servei de Microbiologia

[3] Departament de Patologia i Terapèutica Experimental,Department of Clinical and Molecular Microbiology

[4] Facultat de Medicina i Ciències de la Salut,Dept. of Biological Sciences

[5] Universitat de Barcelona,Laboratorie de Bactériologie, Plateforme de Biologie

[6] University Hospital Centre Zagreb,Division of Hygiene and Medical Microbiology

[7] Munster Technological University,Servicio de Microbiología

[8] Health Services Laboratories,Servei de Microbiologia

[9] Radboud University Medical Centre,CDB, Hospital Clínic de Barcelona

[10] Division of Human Bacterial diseases,ISGlobal, Departament Fonaments Clínics, Facultat de Medicina i Ciències de la Salut

[11] Sciensano,Servicio de Microbiología

[12] Hôpital Cavale Blanche,Laboratorio de Microbiologia

[13] Medical University of Innsbruck,Institut de Microbiologie

[14] Hospital Universitario Central de Asturias,Laboratorio de Microbiología

[15] Universitat de Barcelona,Department of Medical Microbiology

[16] Hospital Universitario A Coruña,undefined

[17] Hospital de la Santa Creu i Sant Pau,undefined

[18] Université de Lausanne,undefined

[19] Biomedical Center,undefined

[20] Faculty of Medicine in Plzen,undefined

[21] Oslo University Hospital,undefined

[22] Hospital Regional de Málaga,undefined

[23] MVZ Dr. Eberhard & Partner Dortmund,undefined

来源：

Scientific Reports | / 12卷

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摘要：

The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2–98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.

引用

共 49 条

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