Complete microbe free processed porcine xenograft for clinical use

被引：5

作者：

Balasundari R. ^{[1
]}

Gupta R. ^{[1
]}

Sivasubramanian V. ^{[1
]}

Chandrasekaran R. ^{[1
]}

Arumugam S. ^{[1
]}

Cherian K.M. ^{[1
]}

Guhathakurta S. ^{[1
,2
]}

机构：

[1] Department of Cardiovascular Research, International Centre for Cardio Thoracic and Vascular Diseases, A unit of Frontier Life Line and Dr. K.M. Cherian Heart Foundation, Mugappair, Chennai

[2] Frontier Life Line, Taramani, Chennai, 600113, Module No: 109, Ticel Biopark

来源：

Indian Journal of Thoracic and Cardiovascular Surgery | 2007年 / 23卷 / 4期

关键词：

Porcine decellularization; Porcine endogenous retrovirus; Xenograft;

D O I：

10.1007/s12055-007-0049-y

中图分类号：

学科分类号：

摘要：

Background: Xenogenic organ replacement from pig to human is a possibility provided sterility and immunogenicity are taken care of. The objective of this study was to check the sterility of processed porcine pulmonary xenografts to mark them 'safe' for clinical application, without the threat for xenozoonoses. Methods: A total of 148 porcine pulmonary valve conduits were screened pre and post processing for bacterial, fungal and viral contamination. The bacterial and fungal contaminants such as Candida species, filamentous fungus, yeast like organisms, gram negative bacteria and porcine endogenous retrovirus (PERV) were isolated from the preprocessed porcine conduits. These harvested conduits were collected in a cocktail of antimicrobials and Hanks balanced salt solution. Next, they were subjected to 'processing' i. e., made completely acellular, strong and biocompatible in all respects. Finally, sterility checks were carried out for bacterial, fungal and viral (PERV) contamination. Result: Microbial evaluation performed in this study revealed that the porcine pulmonary conduits were made 'microbe free' on subjecting to the special staged decellularization technique. Conclusion: The porcine xenograft conduit processed in the laboratory, which has an added advantage of numbers and sizes, can replace the gold standard homograft safely. © 2007 Indian Association of Cardiovascular-Thoracic Surgeons.

引用

页码：240 / 245

页数：5

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