A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins

被引：0

作者：

Lukinavičius G. ^{[1
]}

Umezawa K. ^{[1
]}

Olivier N. ^{[2
]}

Honigmann A. ^{[3
]}

Yang G. ^{[4
]}

Plass T. ^{[5
]}

Mueller V. ^{[3
]}

Reymond L. ^{[1
]}

Corrêa Jr I.R. ^{[6
]}

Luo Z.-G. ^{[7
]}

Schultz C. ^{[5
]}

Lemke E.A. ^{[5
]}

Heppenstall P. ^{[4
]}

Eggeling C. ^{[3
,8
]}

Manley S. ^{[2
]}

Johnsson K. ^{[1
]}

机构：

[1] Ecole Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering (ISIC), National Centre of Competence in Research (NCCR) in Chemical Biology

[2] Ecole Polytechnique Fédérale de Lausanne, Laboratory of Experimental Biophysics, NCCR in Chemical Biology

[3] Max-Planck-Institute for Biophysical Chemistry, Department NanoBiophotonics, 37077 Göttingen

[4] European Molecular Biology Laboratory, Mouse Biology Unit, 00015 Monterotondo (RM)

[5] European Molecular Biology Laboratory, 69117 Heidelberg

[6] New England Biolabs Inc., Ipswich, MA 01938

[7] Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

[8] Medical Research Council Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford

来源：

Nature Chemistry | 2013年 / 5卷 / 2期

基金：

英国医学研究理事会; 欧洲研究理事会;

关键词：

D O I：

10.1038/nchem.1546

中图分类号：

学科分类号：

摘要：

The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging.© 2013 Macmillan Publishers Limited. All rights reserved.

引用

页码：132 / 139

页数：7

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