Production of cello-oligosaccharides from corncob residue by degradation-synthesis reactions

被引：2

作者：

Liang, Yazhe ^{[1
]}

Ji, Wangli ^{[2
]}

Sun, Xianhua ^{[1
]}

Hao, Zhenzhen ^{[1
]}

Wang, Xiaolu ^{[1
]}

Wang, Yuan ^{[1
]}

Zhang, Wei ^{[2
]}

Bai, Yingguo ^{[1
]}

Qin, Xing ^{[1
]}

Luo, Huiying ^{[1
]}

Yao, Bin ^{[1
]}

Su, Xiaoyun ^{[1
]}

Huang, Huoqing ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr & Feeding, 2 West Yuanmingyuan Rd, Beijing 100193, Peoples R China

[2] Chinese Acad Agr Sci, Biotechnol Res Inst, 12 South Zhongguancun St, Beijing 100081, Peoples R China

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2024年 / 108卷 / 01期

基金：

国家重点研发计划;

关键词：

Corncob residue; Cello-oligosaccharides; Cellulase; Phosphorylase; Growth promoter; SUCROSE PHOSPHORYLASE; FUNCTIONAL-CHARACTERIZATION; GROWTH; ENDOGLUCANASE; CELLULASE; STABILITY; ENZYME;

D O I：

10.1007/s00253-023-12832-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The cellulose-rich corncob residue (CCR) is an abundant and renewable agricultural biomass that has been under-exploited. In this study, two strategies were compared for their ability to transform CCR into cello-oligosaccharides (COS). The first strategy employed the use of endo-glucanases. Although selected endo-glucanases from GH9, GH12, GH45, and GH131 could release COS with degrees of polymerization from 2 to 4, the degrading efficiency was low. For the second strategy, first, CCR was efficiently depolymerized to glucose and cellobiose using the cellulase from Trichoderma reesei. Then, using these simple sugars and sucrose as the starting materials, phosphorylases from different microorganisms were combined to generate COS to a level up to 100.3 g/L with different patterns and degrees of polymerization. Using tomato as a model plant, the representative COS obtained from BaSP (a sucrose phosphorylase from Bifidobacterium adolescens), CuCbP (a cellobiose phosphorylase from Cellulomonas uda), and CcCdP (a cellodextrin phosphorylase from Clostridium cellulosi) were shown to be able to promote plant growth. The current study pointed to an approach to make use of CCR for production of the value-added COS.

引用

页码：1 / 11

页数：11

共 53 条

[21] Valorization of waste forest biomass toward the production of cello-oligosaccharides with potential prebiotic activity by utilizing customized enzyme cocktails [J].