Understanding the survival mechanisms of Deinococcus radiodurans against oxidative stress by targeting thioredoxin reductase redox system

被引：0
作者：
Maqbool I. [1 ]
Ponniresan V. [1 ]
Govindasamy K. [1 ,2 ]
Prasad N.R. [1 ]
机构：
[1] Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, 608 002, Tamil Nadu
[2] Dharumapurm Gnanambikai Government Arts College, Dharumapuram Road, Nagapattinam, Mayiladuthurai, 609001, Tamil Nadu
来源：
Archives of Microbiology | 2020年 / 202卷 / 9期
关键词：
Antioxidants; Deinococcus radiodurans; EGCG; Inhibitors; Resistance; Thioredoxin reductase;
D O I：
10.1007/s00203-019-01729-6
中图分类号：
学科分类号：
摘要：
The principal objective of this study is to determine the resistance of Deinococcus radiodurans to hydrogen peroxide (H2O2) induced oxidative stress by inhibiting its thioredoxin reductase (TrxR) antioxidant system. Treatment of D. radiodurans with different TrxR inhibitors such as ebselen, epigallocatechin gallate and auranofin displayed this organism sensitivity to H2O2 treatment in a concentration-dependent manner. We observed that D. radiodurans showed greater resistance to H2O2 treatment. Further, it has also been noticed that TrxR redox system was suppressed by TrxR inhibitors and that this response might be associated with the oxidative stress-mediated cell death in D. radiodurans. Thus, TrxR inhibitors affect the resistance of the D. radiodurans through suppression of its thioredoxin redox pathway via the inhibition of TrxR. Results from this study proved that TrxR plays an important role as an antioxidant enzyme by scavenging intracellular ROS, and thus contributing to the resistance of D. radiodurans towards oxidative stress. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
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页码：2355 / 2366
页数：11
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