Molecular mapping of periodontal tissues using infrared microspectroscopy

被引：17

作者：

Hynes A. ^{[1
]}

Scott D.A. ^{[2
]}

Man A. ^{[3
]}

Singer D.L. ^{[1
]}

Sowa M.G. ^{[3
]}

Liu K.-Z. ^{[3
]}

机构：

[1] Department of Dental Diagnostic and Surgical Sciences, University of Manitoba, Winnipeg, Man.

[2] Oral Health and Systemic Disease Research Group, University of Louisville, School of Dentistry, Louisville, KY 40202

[3] Institute for Biodiagnostics, National Research Council, Winnipeg, Man.

来源：

BMC Medical Imaging | / 5卷 / 1期

关键词：

Chronic Periodontitis; Periodontal Tissue; Spectral Element; Probe Pocket Depth; Clinical Attachment Loss;

D O I：

10.1186/1471-2342-5-2

中图分类号：

学科分类号：

摘要：

Background: Chronic periodontitis is an inflammatory disease of the supporting structures of the teeth. Infrared microspectroscopy has the potential to simultaneously monitor multiple disease markers, including cellular infiltration and collagen catabolism, and hence differentiate diseased and healthy tissues. Therefore our aim was to establish an infrared microspectroscopy methodology with which to analyze and interpret molecular maps defining pathogenic processes in periodontal tissues. Methods: Specific key cellular and connective tissue components were identified by infrared microspectroscopy and using a chemical imaging method. Results: Higher densities of DNA, total protein and lipid were revealed in epithelial tissue, compared to the lower percentage of these components in connective tissue. Collagen-specific tissue mapping by infrared microspectroscopy revealed much higher levels of collagen deposition in the connective tissues compared to that in the epithelium, as would be expected. Thus inflammatory events such as cellular infiltration and collagen deposition and catabolism can be identified by infrared microspectroscopy. Conclusions: These results suggest that infrared microspectroscopy may represent a simple, reagent-free, multi-dimensional tool with which to examine periodontal disease etiology using entirely unprocessed tissue sections. © 2005 Hynes et al., licensee BioMed Central Ltd.

引用

页数：10

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